Engineering a bacterial secretion platform for rapid, high-yield, low-cost production of peptides and proteins for diagnosis and biomedicine

重组蛋白生产是一个价值数十亿欧元的市场，在工业和医药方面有着关键的应用。使用生命所有领域的细胞有机体都可以获得各种生产方法。然而，大多数人依赖于在专用设施中执行的昂贵且耗时的步骤来获得最终的纯蛋白质。该项目旨在通过设计细菌分泌机器来开发可靠而简单的蛋白质生产方法。我们将使用基本和应用两种观点对细菌分泌物进行重新编程，以实现高产量、低成本的体外蛋白质生产。我们将进一步设计这些系统来生产治疗性蛋白质。我们希望这一项目将降低异源蛋白生产的门槛，并在各种领域得到有用的应用，如用于研究的快速成型、个性化药物、在危机情况下低成本/低材料生产医疗蛋白，以及创新的体内疗法的开发。

Recombinant protein production is a multi-billion-euro market with critical applications for industy and medicine. Various methods of production are available using cellular organisms of all domains of life. Yet, most rely on expensive, and time-consuming steps performed in dedicated facilities to obtain the final pure protein. This project aims at developing reliable and simple methods of protein production by engineering bacterial secretion machineries. We will use both fundamental and applied viewpoints to reprogram bacterial secretion for high-yield low-cost in vitro production of proteins. We will further engineer those systems for production of therapeutical proteins. We expect this project to lower the barrier for heterologous production of tailored proteins with useful applications in various fields such as rapid prototyping for research, personalized medicine, low-cost/low-material production of medical proteins in crisis situation, and development of innovative in vivo therapies.