Application of plasma proteins as blood substitutes

Nishi等人。研究了吡哆醇糖化血红蛋白聚氧乙烯醚结合物对肠蠕动的抑制作用、α_1-酸性糖蛋白(α_1-AGP)促进红细胞通过微孔滤器的作用以及α_1-AGP对主动脉血管的扩张作用。发现生理条件下发生的α_1-蛋白酶抑制剂S亚硝化(α_1-PI)对α_1-PI的细胞保护作用的生物学功能存在差异，提示S亚硝化的α_1-AGP可能具有多种生物学功能。纯化的重组人血清白蛋白(RHSA)，由毕赤酵母表达系统分泌，具有与从血清中分离的HSA非常相似的结构特征、稳定性和配体结合特性。分析了来自大肠杆菌的四螺旋束血红素蛋白细胞色素b562结构中辅因子周围的氨基酸残基，并开发了一种在该蛋白中设计外源配体结合口袋的策略。

Nishi et al. found the inhibitory effects of pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) on the intestinal peristaltic movement, the facilitatory effect of α_1-acid glycoprotein (α_1-AGP) on the passage of red blood cells through filters with micropores, and the vasodilator action of α_1-AGP in aorta.Maeda et al. found that S-nitrosylation of α_1-protease inhibitor (α_1-PI) occurring under physiological conditions should diverse in the biological functions contributing to cytoprotective effects of α_1-PI, and suggested that S-nitrosylated α_1-AGP would have the multiple biological functions.Otagiri et al. purified recombinant human serum albumin (rHSA), secreted by a Pichia pastoris expression system, and showed very similar structural characteristics, stability and ligand-binding properties of the rHSA as HSA isolated from sera.Uno et al. analyzed the amino acid residues surrounding the cofactor in the structures of a four-helix bundle heme protein, cytochrome b562 from E. coli, and developed a strategy for designing an exogenous ligand binding pocket into this protein.