Antinociceptive Effect of Central Nerve System with Linear Polarized Near-infrared Light in a Chronic Pain Model

制作了反射性交感神经营养不良的动物模型--Bennett模型。在大鼠右侧坐骨神经周围松散地缠绕4条4-0铬线。左侧坐骨神经不是结扎，而是暴露。在辐射热测试中用热刺激来评估痛觉过敏，用冯·弗雷头发进行压力刺激来评估痛敏。辐射热试验：将大鼠放在玻璃板上。然后，用来自玻璃板下面的辐射热照射鞋底。后爪的收缩会自动终止刺激并停止计时器。压力测试：将大鼠放在金属网架上。在金属网下，鞋底受到使用冯·弗雷头发的压力刺激。移动后爪的细丝的厚度是痛觉过敏的一个指标。选择术后一周出现肥大反应的大鼠。采用线偏振近红外光单次照射延髓和大脑感觉区，观察其抗伤害性作用。大约8周后，Bennett模型的痛觉过敏消失。选择术后1周出现肥大反应的大鼠。我们每天在延髓附近用线偏振近红外光照射一到三周。我们每天都在大脑感觉区附近用光照射一到三周。我们考察了它是否具有恢复作用。Shimose制作了动物模型，并照射了线偏振近红外光。Muneshige做了辐射热测试，Shimose做了von Frey头发测试。预期结果：在RSD动物模型上，用光照射延髓或大脑感觉区附近具有抗伤害性作用。这表明人类大脑感觉区和延髓附近的光照射是可能的。

Bennett model which is regarded as an animal model of RSD (Reflex sympathetic dystrophy) is made. Four 4-0 chromic gut sutures were tied loosely around the right sciatic nerve of the rat. The left sciatic nerve is not ligated but exposed. The hyperalgesia is evaluated with the thermal stimulation in a radiant heat test, and is done with the pressure stimulation using a von Frey hair. Radiant heat test : The rat is put on the glass plate. Then, the sole is irradiated with radiant heat from under the glass plate. Hind paw withdrawal automatically terminated the stimulus and stop the timer. Pressure test : The rat is put on the metal mesh. From under the metal mesh, the sole is stimulated with the pressure stimulation using a von Frey hair. The thickness of the filament which moves the hind paw is an index of hyperalgesia. Rats that showed hyperlgesia one week postoperatively are chosen. Single-session irradiation with linear polarized near infrared light was made near the medulla oblongata and the cerebrum sensory area, and we observe the antinociceptive effect. The hyperalgesia of the Bennett model disappears about eight week later. Rats that showed hyperlgesia one week postoperatively were chosen. We irradiates every day near the medulla oblongata for one or three weeks with linear polarized near-infrared light. We irradiates every day near the cerebrum sensory area for one or three weeks with the light. We examined whether it has recovery effect or not. Shimose makes the animal model and irradiates linear polarized near-infrared light. Muneshige makes the radiant heat test, and Shimose does the von Frey hair test. The anticipating result : Irradiation near the medulla oblongata or cerebrum sensory area with the light showed antinociceptive effect in the animal model of RSD. This shows the possibility of the effect of the light irradiation near cerebrum sensory area and medulla oblongata in the human being.