BIOMECHANICAL COMPARISON BETWEEN EFFECTS OF STRESS-ENHACEMENT AND STRESS-DEPERIVATION ON REMODELING OF PATELLAR TENDONS AFTER THE IN-SITU FREEZE-THAW TREATMENT

1. 抑制外源性细胞浸润对内源性成纤维细胞坏死后髌骨肌腱力学性能的影响：我们用72只家兔分析了抑制外源性细胞浸润对内源性成纤维细胞坏死后髌骨肌腱力学性能的影响。结果，外源性细胞浸润的抑制阻止了内源性细胞坏死后髌骨肌腱力学性能的降低。应力增强对原位冻融髌骨肌腱力学性能的影响：采用30只家兔进行生物力学研究，探讨应力增强对原位冻融髌骨肌腱力学性能的影响。结果，过高的应力降低了冻融髌骨肌腱的力学性能。内源性成纤维细胞和外源性浸润细胞对应力增强髌骨肌腱细胞外基质力学性能的影响采用48只家兔，研究内源性成纤维细胞和外源性浸润细胞对应力增强髌骨肌腱细胞外基质的影响。结果，内源性成纤维细胞阻止应力增强对髌骨肌腱力学性能的不利影响，外源性浸润细胞增加髌骨肌腱的横截面积。应力增强和应力剥夺对冻融髌腱力学性能影响的生物力学比较：在兔模型中，我们比较了应力增强和应力剥夺对冻融髌腱力学性能的影响。结果表明，应力剥夺使冻融髌腱的力学性能迅速恶化，而应力增强则使其逐渐恶化。结论：本项目揭示了应力增强对冻融髌骨肌腱力学性能的影响不同于应力剥夺，外源性浸润细胞的生物学反应参与了这些影响的机制。少

1. Effects of Inhibition Extrinsic Cell Infiltration on Patellar Tendons After Intrinsic Fibroblast Necrosis : We analyzed the effect of inhibition of extrinsic cell infiltration on mechanical properties of the patellar tendon after intrinsic fibroblast necrosis using 72 rabbits. As a result, the inhibition of extrinsic cell infiltration prohibited the reduction in mechanical properties of the patellar tendon that occurred following intrinsic cell necrosis.2. Effects of Stress Enhancement on the Mechanical Properties of in situ Frozen Patellar Tendons : A biomechanical study was conducted to clarify the effect of stress enhancement on the mechanical properties of the in situ frozen-thawed patellar tendons using 30 rabbits. As a result, excessively high stress reduced the mechanical properties of the frozen-thawed patellar tendon.3. Effects of the Presence Intrinsic Fibroblasts or Extrinsic Infiltrating Cells on the Mechanical Properties of the Extracellular Matrix in the Stress-enhance … More d Patellar Tendon A study was conducted to determine the effect of the presence of intrinsic fibroblasts or extrinsic infiltrating cells on the extracellular matrix of the stress-enhanced patellar tendon using 48 rabbits. As a result, intrinsic fibroblasts prohibited detrimental effects of stress enhancement on mechanical properties of the patellar tendon and extrinsic infiltrating cells increased the cross-sectional area of the patellar tendon.4. Biomechanical Comparison between Effects of Stress-enhancement and Stress-deprivation on Mechanical Properties of Frozen-thawed Paterllar Tendons : We compared effects of stress-enhancement on mechanical properties of the frozen-thawed patellar tendon to those of stress-deprivation in a rabbit model. As results, stress-deprivation rapidly deteriorated mechanical properties of the frozen-thawed patellar tendon, while stress-enhancement gradually deteriorated them.5. Conclusions : This project has revealed that effects of stress-enhancement on mechanical properties of the frozen-thawed patellar tendon are different from those of stress-deprivation, and that biological responses of extrinsic infiltrating cells contribute to mechanisms of these effects. Less