Fiber-optic approaches for diagnosis of gastrointestinal disease

为了阐明第二代光敏剂单l -天冬氨酸氯e6（NPe6）的有效性，我们在小鼠模型中研究了光动力诊断和光动力治疗肠道肿瘤的可能性。(1)在给予光敏剂后，分别用664 nm二极管激光对肠道肿瘤部位进行恒定间隔照射。(2)切除肿瘤，冷冻切片，显微镜下观察荧光。观察荧光图像，计算肿瘤在6小时内的平均荧光强度明显高于正常粘膜（P<0.001）。NPe6的荧光在显微荧光图像上主要以红色荧光形式积聚在肠道肿瘤中。我们认为NPe6可能是一种有价值的光敏剂，可用于肠道肿瘤的光动力诊断和光动力治疗。我们的主要目标是基于电子调谐钛蓝宝石激光器的拉曼光谱应用和先进光谱工具的发展，为生物医学研究开发先进的分析技术。

To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6(NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model. (1) The intestinal tumor sites were irradiated with a 664-nm diode laser constant intervals after the administration of photosensitizers, respectively. (2) The tumors were excised and processed for the frozen section to observe the fluorescence by a microscope. We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (P<0.001). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image. We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors.Our major goal is to develop advanced analytical techniques for biomedical researches based on the Raman spectroscopic applications of an electronically tuned Ti:sapphire laser and the developments of advanced spectroscopic tools.