Analysis of peptide hormone receptors in vetebrate reproductive endocrine system

本研究的目的是通过对生殖相关激素及其受体的表征来了解脊椎动物生殖内分泌系统的进化史。在这项研究中，我们建立了爬行动物的繁殖群体，并表征了一种新的GnRH生理功能。豹纹壁虎（Eublephalis macularius）繁殖群体作为爬行动物模型动物的建立：为了促进爬行动物生殖内分泌系统的系统研究，我们尝试从多种爬行动物中选择实验动物模型。其中，豹壁虎因其独特的性别决定系统和易于操作而最适合。现在，我们的豹壁虎种群已经繁殖了4年，目前的种群规模约为70头。鸟类和爬行动物生殖相关激素受体的分子特征：利用新建立的豹壁虎繁殖群体，我们对类固醇激素受体（更多的是雌激素、雄激素和孕激素受体）和GnRH受体进行了表征。到目前为止，这些受体的部分cDNA序列已被确定，它们的完整序列为GnRH受体。到目前为止，这些受体的部分cDNA序列已经被鉴定，它们的完整序列正在通过RACE和配体结合活性进行鉴定。调节GnRH集落形成效率的活性：GnRH促进垂体分泌GTH。然而，越来越多的证据表明垂体外作用对周围组织的数量有影响。在本研究中，我们发现GnRH对菌落形成效率的调节具有很高的敏感性（10^<-15> M -10^<-12>M）。这种活性也受到细胞系自身和/或旁分泌活性的极大影响。豚鼠GnRH受体的分子特征：在所有已知的GnRH分子形式中，豚鼠GnRH被预测具有独特的结构。在本研究中，我们对豚鼠垂体中GnRH受体的mRNA进行了表征。表征的GnRH受体属于I型GnRH受体组，具有与大鼠GnRH受体相似的配体结合特性。少

The purpose of this research is to understand the evolutionary history of the reproductive endocrine system of vertebrates by characterization of reproduction-related homones and their receptors. During this study we have established breeding colony of a reptile, and characterized a novel GnRH physiological function.1. Establishment of breeding colony of Leopard Gecko (Eublephalis macularius) as a reptilian model animal : To promote systematic research in reptilian reproductive endocrine syste, we have tried to select from various reptile species for a loboratory animal model. Among them, leopard gecko was most suitable because of their unique sex determinating system and easy handling. Now, our leopard gecko colony has been bred for 4 years and the current colony size is about 70 heads.2. Molecular characterization of Reproduction related hormone receptor in birds and reptiles : Using our newly established leopard gecko breeding colony, we have characterize steroid hormone receptors ( … More estrogen, androgen and progesterone receptors) and GnRH receptor. So far, partial cDNA sequences of these receptors are characterized, and their full sequences are GnRH receptor. So far, partial cDNA sequences of these receptors are characterized, and their full sequences are under the characterization by RACE and ligand binding activity.3. Regulating activity of colony-forming efficiency of GnRH : GnRH promotes GTH secretion from pituitary. However there is increasing evidence for extra-pituitary effects on number of peripheral tissues. In this study, we found that GnRH regulates the colony forming efficiency with high secsitivity (10^<-15>-10^<-12>M). This activity was also greatly effected by auto-and/or paracrine activity of the cell line.4. Molecular characterization of Guinea pig GnRH receptor : Guinea pig GnRH is predicted to have a unique structure among all known forms of GnRH molecule. In this study, we characterized mRNA of GnRH receptor from the guinea pig pituitary. Characterized GnRH receptor is belong to type I GnRH receptor group, and shows similar ligand binding characteristics to that of the rat GnRH receptor. Less