Localigation oi P.acnes in sarcoid granumoma and identification of a liability antigen in P.acnes cell-wall component

尽管对结节病的发病机制进行了广泛的研究，但结节病的病因仍不清楚。在之前的研究中，Honma H.和他的同事在结节病患者的淋巴结中发现了痤疮丙酸杆菌（P. acnes）。在这项研究中，我们研究了在结节病患者支气管肺泡灌洗（BAL）中恢复的细胞中检测痤疮假单胞杆菌的DNA。我们采用巢式聚合酶链反应（PCR）和原位PCR杂交作为检测痤疮假单胞杆菌DNA的工具。pcr技术是基于痤疮假单胞杆菌小亚基核糖体RNA的扩增。对30例组织学证实的结节病患者和30例其他肺部疾病患者的BAL细胞进行特异性检测，检测方法为痤疮假单胞杆菌DNA。结节病患者BAL细胞中检测到痤疮p . DNA的比例为70%，对照组为23.3%，结节病的比例显著高于对照组（p<0.001）。原位PCR杂交发现，痤疮假单胞杆菌DNA信号仅在结节病患者的肺泡巨噬细胞细胞质中检测到，而在非结节病患者的肺泡巨噬细胞中检测不到。

Despite the extensive investigations into the pathogenesis of sarcoidosis, the etiology of sarcoidosis is still unknown. In previous studies, Honma H. and his colleagues found Propionibacterium acnes (P. acnes) in the affected lymph nodes of sarcoidosis patients. In this study, we investigated to detect DNA of P. acnes in cells recovered from bronchoalveolar lavage (BAL) in patients with sarcoidosis. We applied a nested polymerase chain reaction (PCR) assay and in situ PCR hybridization as a tool to detect DNA of P. acnes. The PCR-technique is based on the amplification of P. acnes small subunit ribosomal RNA. BAL cells from thirty patients with histologically proven sarcoidosis and 30 controls with other pulmonary diseases were underwent assay by specifically detected DNA from P. acnes. DNA of P. acnes was detected in 70% in the BAL cells with sarcoidosis patients and 23.3% in the controls, the proportion of sarcoidosis is significantly greater than that of controls (p<0.001). By in situ PCR hybridization, signal of P. acnes DNA was detected only in cytoplasm of alveolar macrophages from sarcoidosis, but not from non-sarcoidosis patients.