The study on significance of imprinting gene(s) for the development of insulin-dependent diabetes mellitus in children

我们对儿童1型糖尿病一级亲属的糖尿病家族史进行了研究。我们发现，在进展缓慢的1型糖尿病患者中，糖尿病家族史明显高于急性发作型糖尿病患者。此外，母亲的糖尿病发病率显著高于父亲。为了探究1型糖尿病易感基因印迹等表观遗传机制的可能性，我们提取了1型糖尿病患儿的父母和兄弟姐妹的DNA。本研究经北内民族委员会批准。20对父母和兄弟姐妹同意参与他的研究。我们分析了1型糖尿病患儿及其父母和兄弟姐妹的HLA - DQ-β、-α基因、DR-β基因、CTLA-4基因、NeuroD/BETA2基因、IDDHK1、2-22基因的易感基因是否来源于母亲或父亲。父母的绝对数量太少，不能得出结论，在1型糖尿病儿童中，易感HLA基因来自父亲多于来自母亲。

We have studied the family history of diabetes mellitus among first degree relatives of type 1 diabetes mellitus in children. We found the family history of diabetes was significantly higher in slowly progressing type 1 diabetes than acutely onset diabetes. Moreover, incidence of mother's diabetes was significantly higher than that of father. To role out the possibility of epigenetic mechanism, such as imprinting of susceptibility gene for type 1 diabetes, we extracted DNA from parents and sibling of type 1 diabetic children. This study was approved by the B Kitasato Ethnic committee. Twenty set of parents and sibling were agreed to joint his study. We have analyzed HLA DQ-β, -α gene, DR-β gene, CTLA-4 gene, NeuroD/BETA2 gene, IDDHK1, 2-22 gene in children with type 1 diabetes and their parents and sibling whether susceptibility genes derived from mother or father. The absolute number of parents were too small to draw conclusions, susceptible HLA genes derived more from the father than the mother in type 1 diabetic children.