Involvement of FSP1 positive fibroblasts in the progression of human glomerulonephntis

采用抗FSP1多克隆抗体免疫组化方法研究肾组织中成纤维细胞特异性蛋白1 （FSP1）的定位。对320例不同类型肾小球肾炎患者（IgA肾病142例，膜性肾病28例，局灶性及节段性肾小球硬化36例，微小改变肾病综合征31例，狼疮性肾炎41例，月牙状肾小球肾炎42例）进行肾活检。FSP1阳性成纤维细胞聚集在显示严重间质纤维化的区域。纤维化区部分小管上皮细胞也表达FSP1。FSP1阳性细胞数与纤维化面积呈正相关，采用标准点计数法测定（r = 0.84, p < 0.0001）。FSP1阳性细胞数与血清肌酐呈正相关（r = 0.67, p < 0.0001），与肌酐清除率呈负相关（r = -0.71, p < 0.0001）。我们还检测了肾小球FSP1的表达。在月牙状肾小球肾炎患者中，FSP1定位于内脏上皮细胞和月牙。局灶性和节段性肾小球硬化患者内脏上皮细胞中FSP1的表达水平明显高于轻度改变肾病综合征患者。

Localization of fibroblast specific protein 1 (FSP1) in kidney was studied by immunohistochemistry using anti-FSP1 polyclonal antibody. Renal biopsy specimens were obtained from 320 patients with various kinds of glomerulonephritis (IgA nephropathy, 142 patients ; membranous nephropathy, 28 patients; focal and segmental glomeruloscrelosis, 36 patients; minimal change nephritic syndrome, 31 patients ; lupus nephritis, 41 patients; crescentic glomerulonephritis 42 patients). FSP1 positive fibroblasts accumulated in the area showing severe interstitial fibrosis. Some tubular epithelial cells in the fibrosis area also expressed FSP1. The number of FSP1 positive cells was positively correlated with the area of fibrosis, which was measured using standard point-counting method (r = 0.84, p < 0.0001). The number of FSP1 positive cells was positively correlated with serum creatinine (r = 0.67, p < 0.0001) and negatively correlated with creatinine clearance (r = -0.71, p < 0.0001).We also examined glomerular FSP1 expression. In patients with crescentic glomerulonephritis, FSP1 was localized in visceral epithelial cells and in crescents. Levels of FSP1 expression in visceral epithelial cells in patients with focal and segmental glomeruloscrelosis was significantly higher than those in patients with minimal change nephritic syndrome.