What kind of food protein remarkably enhances GFR in man

Hostetter报告称，健康受试者摄入200克牛排会导致大约120%的GFR升高。但是，还没有研究使用等量的植物蛋白。大多数研究表明，原料在烹调前的氨基酸组成，以及氨基酸的分析方法和浓度的指示是模糊的。我们尝试在相同的6名年轻男性受试者获得知情同意的情况下，在急性负荷相同数量的不同蛋白质(牛排或烘焙脱脂大豆)的情况下测量GFR(Gin)。每个受试者都接受了饥饿、摄入牛排或烘焙脱脂大豆。启动、牛排或大豆交替摄入，间隔一周。牛排和烘焙脱脂大豆中的蛋白质含量都调整为64.5g。除色氨酸(氢氧化钠水解)外，其余氨基酸用盐酸水解法和高效液相色谱(HPLC法)分析。以饥饿状态下的GFR为对照。纯菊粉由富士制药公司提供。清除性试验采用Wessons法。采用Jaffe速率法测定Ccr。同时测定血浆胰高血糖素和尿c-GMP。统计学检验采用学生检验。牛排的杜松子酒和烘烤脱脂大豆的杜松子酒高于饥饿阶段。不同时期的牛排和烤脱脂大豆之间差异不显著。与饥饿阶段相比，Ccr在两个阶段的升高更为显着。三组大鼠血浆胰高血糖素升高的方式相似。但尿c-GMP排泄量无明显增加。牛排和脱脂大豆中丙氨酸、甘氨酸和精氨酸的总量约为13.5g。我们的研究表明，植物蛋白(大豆)可以与动物(牛肉)蛋白一样提高GFR。

Hostetter has reported 200g of beefsteak ingestion in healthy human subjects caused about 120% elevation of GFR. But, there have been no studies using the same amount of vegetable protein. Almost study indicated the amino acid composition of raw materials before cooking and the analytical methods and the indication of the concentration of amino acids are obscure. We have attempted GFR (Gin) measurement under acute loading of the same amount of different protein (beefsteak or baked defatted soybean) in the same six young male subjects obtained informed consent. Each subject has been received starvation, ingestion of beefsteak or baked defatted soybean.Starvation, beefsteak ingestion or soybean ingestion alternatively with interval of one week.The amount of protein was adjusted 64.5g both in beefsteak and baked deffated soybean. The amino acid composition was analysed with HC1 hydrolysis and HPLC except tryptohan(NaOH hydrolysis). GFR in the starvation was considered as control. Pure inulin was supplied by Fuji Pharmaceut.Co. Clearance test was performed by Wessons method. Creatinine clearance (Ccr) was determined by Jaffe rate assay method. Plasma glucagon and urinary c-GMP were also measured. Statistical significance was checked by Student test. Gin of beefsteak and those of baked defatted soybean stages elevated compared to that of starvation stage. No significant difference was observed among beefsteak and baked deffated soybean stage. Ccr elevated in both stages compared to starvation stage more remarkably. Plasma glucagon elevated a similar manner in three groups. But no significant increase in urinary excretion of c-GMP. Total amount of alanine, glycine and arginine was about 13.5g both in beefsteak and baked defatted soybean. Our study revealed that vegetable protein (soybean) could elevates GFR as high as animal (beef) protein.