Difference of proliferation activity in animal and human vascular cells

背景资料：人工血管的内皮化在动物模型中很常见，而人工血管移植物中内皮细胞覆盖的情况非常罕见，并且涤纶人工血管移植物能否实现内皮覆盖尚不清楚。目的：探讨不同动物和年龄段血管细胞增殖活性的差异。材料与方法：分离培养SD大鼠下腔静脉、犬颈静脉和人大隐静脉的内皮细胞（EC）和平滑肌细胞（SMCs），用Almar Blue法检测其增殖活性。大鼠和狗的内皮细胞生长至50%汇合的时间分别为4天和8天，而人的内皮细胞在2周内没有增殖汇合。大鼠、犬和人中SMC生长至50%汇合的时间分别为3天、9天和15天。结论：内皮细胞和平滑肌细胞的增殖活性随增龄而降低，但人的增殖活性下降幅度更大。这可能是由于细胞增殖活性的差异，人类和动物之间的差异，只是由于人口倍增。

Background: Endothelialization of vascular prosthesis is common to animal models, whereas it is extremely rare that prosthetic grafts implanted in human subjects is lined by endothelial cells (ECs), and it remains unclear whether Dacron grafts to human subjects can achieve endothelial coverage.Objective: We investigate the differences of proliferation activity of vascular cells with animal species and aging.Materials and Methods: ECs and smooth muscle cells (SMCs) isolated from inferior vena cavas of Sprague-Dawley rat, canine jugular veins, and human saphenous veins, were cultured and their proliferation activities were measured by Almar Blue assay.Results: The ECs and SMCs proliferation were dominant to the order: rat, dogs, and human being. The times to grow 50 % confluency of ECs in the rats and dogs were 4 days and 8 days,but human ECs did not proliferate confluentry in 2 weeks. The times to grow 50 % confluency of SMCs in the rats, dogs, and human were 3 days, 9 days and 15 days. Though ECs and SMCs proliferation activity decreased with aging, that of human being decreased much more.Conclusion: Proliferation activity differed among the animals and decreased with aging. It might be that the differences in cell proliferation activities between human and animals due to only the differences of population doubling.