Functional modulation and control of osteoclastgenesis by wear debris and inflammatory cytokines.

为阐明钛颗粒对成骨细胞活性和细胞增殖的影响，本实验对两种人成骨细胞(MG-63和SaOS2)和人成骨细胞在钛颗粒存在或不存在的情况下进行了培养。随着微粒浓度的增加，细胞存活率和细胞增殖能力下降。这些结果提示钛颗粒可能会减少种植体周围的骨形成。II.为了阐明钛颗粒在成骨细胞MMPs-TIMPs系统中的作用，进行了定量PCR分析。结果提示，钛颗粒可能通过1)减少骨形成，2)促进破骨细胞性骨吸收，从而促进破骨细胞在骨表面附着。

I. To clarify the role of titanium particles on osteoblast viability and cell proliferation, two human osteoblastic cells (MG-63 and SaOS2) and human osteoblasts were cultured in the presence or absence of titanium particles. The viability and cell proliferation were deteriolated as concentrations of the partikles increased. These results suggest that titanium particles may reduce the bone formation arround implants.II. To clarify the role of titanium particles on osteoblast MMPs-TIMPs system, quantitative PCR analysis was performed. In early time exposure, MMP-1 production was increased, and after long time exposure, MMP-1, -2, and TIMP-1, -2 production in ribonucleic acid levels were suppressed.This data suggested that titanium particles between bone and implants may provide the space of osteoclast attachment on bone surface to enhance osteoclastic bone resorption.These results suggest that titanium particles generated between bone and implants may play an important role for bone resorption through 1) reduction of bone formation and 2) accerelate osteoclastic bone resorption.