Gene TheraphyforSpinal Cord Injury

脊髓损伤急性期高压氧治疗对iNO和GDNF的影响。为了研究高压氧通气（HBO）对脊髓损伤的急性影响。我们采用逆转录酶-聚合酶链反应（RT-PCR）检测GDNF mRNA和iNOSmRNA。脊髓损伤后30分钟内HBO有效，但30分钟后HBO有效，而不是次数，脊髓损伤后胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor，GDNF）的表达上调我们采用大鼠脊髓急性挫伤后，用失重法和逆转录-聚合酶链反应（RT-PCR）研究了GDNF基因的时空表达模式。结果显示，GDNF在脊髓损伤后30 min内开始转录增加，并在3 h内达到高峰。免疫组化结果显示GDNF主要表达于伤后1d的小胶质细胞和肢端胶质细胞，而神经元和星形胶质细胞未见表达。GDNF基因表达的这种立即上调可能是炎症过程的一个组成部分，并可能对脊髓损伤（SCI）后的神经元产生保护作用。

Elect of hyperbaric oxygen therapy on iNOand GDNF in the acute phase of spinal cord injury.To study the acute effect of hyperbaric oxygen ventilation (HBO) on spinal cord injury.We examined GDNFmRNA and iNOSmRNA using Reverse transcriptase-polymerase chain reaction (RT-PCR).HBO was effective within 30minitsu after spinal cord injury but the HBO after 30minitsu, instead of the times, was not effective.Up-regulation of glial cell line-derived neurotrophic factor(GDNF) following traumatic spinal cord injury.We investigated the temporal and spatial expression patterns of the GDNF gene after subjecting rats to an acute contusion injury of the spinal cord using the weight drop technique Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that GDNF transcription in the spinal cord began to increase within 30 min after injury and peaked within 3h. Immunohistochemical analysis showed GDNF immunoreactivity to be present mainly in microglia and acrophages 1 day after injury, but not in the neurons or astrocytes. This immediate upreguration of GDNF gene expression may be a component of an inflammatory process and probably exerts aprotective effect on neurons following apinal cord injury(SCI).