Effect of Heart Shock Protein and Interferon on Toxoplasmic Chorioretinitis

1. 探讨干扰素(IFN)-γ对刚地弓形虫囊炎经口感染后器官感染性、弓形虫绒毛膜视网膜炎及易感（C57BL/6）和耐药（BALB/c）菌株遗传易感性的影响。采用C57BL/6和BALB/c背景下IFN-γ敲除（KO）小鼠。采用竞争性聚合酶链反应法定量评价了经口感染后不同时间在包括眼睛在内的不同器官内弓形虫丰度变化的动力学。在IFN-γ KO小鼠中，在所有检查的器官中都检测到弓形虫特异性基因SAG1，并且原生动物的增殖比野生型小鼠活跃得多。弓形虫在肠系膜淋巴结和心脏的丰度明显高于其他器官。相反，在神经系统器官和肾脏中，只观察到微弱的可检测的反应。在感染过程中，刚地弓形虫在IFN-γ KO C57BL/6小鼠的器官中比在IFN-γ KO BALB/c小鼠的器官中生长得更快。破坏INF-γ对易感和耐药小鼠的感染性均有显著影响。弓形虫HSP70的高表达与感染弓形虫的IFN-γ KO小鼠的死亡率相关。在感染弓形虫的IFN-γ KO小鼠眼内检测到SAG1。

1. To determine the influence of interferon (IFN)-γ on the organ infectivity, on the Toxoplasmic chorioretinitis, and on the genetic susceptibility of susceptible (C57BL/6) and resistant (BALB/c) strains after peroral infection with cystis of Toxoplasma gondii. IFN-γ knockout (KO) mice in C57BL/6 and BALB/c backbrounds were utilized. The kinetics of the changes in T.gondii abundance were evaluated with a quantitative competitive polymerase chain reaction assay in various organs including eyes at different times after peroral infection. In IFN-γ KO mice, a T.gondii-specific gene, SAG1, was detected in all organs examined, and the protozoan proliferated much more actively than in wild-type mice. The abundance of T.gondii was much higher in mesenteric lymph nodes and the heart than in other organs. In contrast, in the nervous system organs and kidneys, only a weakly detectable reaction was observed. Toxoplasma gondii grew at a more rapid rate in the organs of IFN-γ KO C57BL/6 mice than in the organs of IFN-γ KO BALB/c mice during the course of infection. Destruction of the INF-γ showed remarkable effects on the infectivity in both susceptible and resistant mice.2. The high-level expression of gondii HSP70 was correlated with mortality in IFN-γ KO mice infected with T.gondii.3. SAG1 was detected in the eye of IFN-γ KO mice infected with T.gondii perollary.