Search for the expression of estrogenic action of dental materials

最近的研究表明，一种雌激素化学物质双酚A（BPA）可能会从牙科密封剂中滤出。本研究旨在体外鉴定和定量密封剂和牙科聚碳酸酯材料中释放的BPA和其他组分。通过液相色谱分析，测试的密封剂没有显示出释放BPA。三种牙科聚碳酸酯材料每克颗粒含有28.8至203 μg BPA。而用PBS（-）提取的牙科PC材料中BPA的含量低于μg/g牙科PC材料。雌激素敏感的MCF-7乳腺癌细胞是一个有用的工具，可以估计一种雌激素化学物质（环境雌激素、外源雌激素）。结果表明，BPA、17 β-雌二醇、己烯雌酚（DES）和炔雌醇对MCF-7细胞增殖有促进作用，并诱导孕酮受体mRNA的表达。含PBD（-）的牙用封闭剂和PC材料提取液对MCF-7细胞增殖和孕酮受体表达无影响。

Recent studies have been saised about the possibility that one of estrogenic chemicals, bisphenol A (BPA) might be leached out of dental sealants. This study aimed to identify and quantify BPA and other components released from sealants and dental polycarbonate materials in vitro. The sealants tested were not shown to have released BPA analyzed by a liquid chromatography. The three dental polycarbonate materials contained the amounts of 28.8 to 203 μg of BPA pre gram of pellet. But the content of BPA of dental PC material extracted with PBS(-) was less than μg level per gram dental PC material.The estrogen-sensitive MCF-7 breast canser cells was a useful tool that may estimate an estrogenic chemicals (environmental estrogens, xenoestrogens). When incubated with MCF-7 cultured with dextran-charcoal treated FCS and phenol red-free medium, BPA, 17 β-estradiol, diethylstilbestrol (DES) and ethynylestradiol promoted cell proliferation that was assayed the DNA content by DABA method, and induced the expression of progesterone receptor mRNA that was analyzed by RT-PCR. The extracts of dental sealants and dental PC materials with PBD(-) were not affected the proliferation and the expression of progesterone receptor of MCF-7.