Bacteriorogical Study for Conservative Treatment of Pulp-Temporary Indirect Plup Capping-

在本实验中，对以冷水疼痛为主的牙髓病牙齿进行了临床检查，并在蛀牙和牙髓的牙本质深处发现了细菌。因此，总结如下。1. 所有病例均从牙本质深层分离出细菌。结果发现，当牙髓室打开时，细菌可以被带到牙髓管。因此，在所有因龋齿引起的牙髓疾病而采用牙髓切除治疗的病例中，在填充根管之前对根管进行细菌培养的必要性有所不同。2. 在所有从根管中分离出的细菌种类较少的情况下，分离出的细菌与从牙本质深层检测到的细菌相同，这表明存在于牙本质深处的细菌直接侵入牙髓而影响牙髓。3. 在根管中分离的细菌种类较多的情况下，非从牙本质深层分离的专性厌氧细菌如Bacteroides、Fusobacterium、Prevotella和专性厌氧乳酸杆菌的分离率较高，从牙髓中检出的细菌数量较多。这表明，当细菌从牙本质深处侵入到牙髓内繁殖时，它们可能会改变类型和百分比。4. 如果蛀牙的牙本质是潮湿的、深褐色的，细菌侵入牙髓的可能性比较高，这可以为确定治疗措施提供指导

In this experiment, teeth with dental pulp disease, mainlyexperiencing cold water pain, have been clinically examined and bacteria has been identified from deep in the dentin of decayed teeth and dental pulp. Accordingly, the following are concluded. 1. Bacteria were isolated from the deep part of dentin in allcases examinaed. It was found that the bacteria could be carried to the canal while the pulp chamber is open. Thus the need for bacteriologic culturing of root canals before the canal is filled for all cases in which pulpectomy treatment has been applied to dental pulp desease caused by caries has been varified. 2. In all the cases wherein the number of bacterium types isolated from the canal was small, the isolated bacteria were identical to some of those detected from the deep part of dentin, which implies that dental pulp is affected by direct invasion into it by bacteria existing deep in dentin. 3. In instances where the number of bacterium types isolated from canal was large, obligate anaerobic such as Bacteroides, Fusobacterium, Prevotella and obligate anaerobic Lactobacillus, which were not isolated from the deep part of dentin, had a comparatively high ratio of isolation and the number of bacteria detected from the dental pulp proved to be large. This suggests the possibility that, at the stage when bacteria invading from the deep part of dentin multiply within the dental pulp, they change types and the percentages. 4. In case of a decayed tooth with a damp, dark-brown infected dentin, the possibility of bacteria invasion into the dental pulp to be relatively high, which can furnish a guideline for determining treatment measures