Analysis of abnormal DNA structure and down regulation of a new tumor suppressor, FEZ1 gene, in oral squamous cell carcinoma.

8号染色体(8p)短臂上的等位基因缺失在包括口腔癌在内的几种人类恶性肿瘤中是常见的。我们在口腔鳞状细胞癌S中发现了两个常见的8p(8p2，8p22)缺失区域，并探讨了位于8p22的候选抑癌基因FEZ1/LZTS1(FEZ1)的可能参与。在这里，我们分析了FEZ1基因改变是否在口腔鳞状细胞癌的发生和发展中起作用。在本研究中，我们用逆转录聚合酶链式反应(RT-PCR)检测了31例原代口腔鳞状细胞癌和8例口腔鳞癌来源细胞系中FEZ1的表达。35%的肿瘤(11/31)和100%的细胞株(8/8)的mRNA基因表达缺失或降低。此外，为了探讨沉默的机制，用5-氮杂-2‘-脱氧胞苷培养细胞，所有细胞系都被去甲基化试剂修复。这些发现提示，FEZ1基因失活可能与口腔鳞癌的发生有关。

Allelic deletions on the short arm of chromosome 8 (8p) are frequent events inseveral human malignancies, including oral cancer. We have examined and found two common regions of deletion on 8p (8pl2, 8p22) in oral squamous cell carcinomas (SCC) s. The possible involvement of FEZl/LZTSl (FEZ1) gene, a candidate tumor suppressor gene, mapped at 8p22, was also evaluated. Here we analyzed whether FEZl alterations play arole in the development and progression of oral SCCs. In present study, we examined FEZ1 expression in 31 primary oral SCCs and 8 SCC-derived cell line by reverse transcription-PCR (RT-PCR). Thirty-five % of tumor (ll of 31) and 100% of cell lines (8 of 8) showed absent or reduced mRNA gene expression. Furthermore, to investigate the mechanism for silencing, cell were cultured with 5-aza-2'-deoxycytidine and all of cell linesshowed restoration by demethylating agent. These findings suggest that inactivation of the FEZ1 gene may contribute to the development of oral SCCs.