STUDY OF TUM0R SUPPRESSOR GENE AND ANGIOGENESIS IN ORAL CAJNCER -Effect of the transfection of p53 and p16 tumor suppressor genes on angiogenesis-.

1)抑癌基因P53和p16在血管生成中的作用作为SCC细胞系的结果，HSC-4外显子2的R74(CGA)突变为终止密码子(TGA)，外显子2/3的剪接受体缺失检测到SCC-4。2)血管生成因子和抑癌基因在口腔鳞状细胞癌中的表达及其临床病理意义。用CD31抗体阳性微血管数评价瘤内微血管密度。结果：VEGF-C的表达与淋巴结转移有关(p&lt；0.01)。Logistic逐步回归分析显示，单因素分析显示淋巴结转移与肿瘤侵袭方式、VEGF-C表达及血管侵犯有关(p&lt；0.05)。多因素分析显示，侵袭方式和VEGF-C表达是影响淋巴结转移的独立因素(p&lt；0.05)。PD-ECGF与瘤内微血管密度呈正相关(p&lt；0.05)。在Cox多因素比例风险模型中，VEGF、肿瘤分化程度、侵袭方式和淋巴结转移是影响预后的独立因素(p&lt；0.05)。结论：口腔鳞癌组织中VEGF、PD-ECGF和VEGF-C的表达分别与预后、血管生成和淋巴结转移有关。而抑癌基因p53和p16与肿瘤转移和预后无关。

I) Effect of tumor suppressor gene p53 and p16 on angiogenesisAs a result of SCC cell lines, HSC-4 was mutated R74 (CGA) to end codon (TGA) in exon 2 and SCC-4 was detected in the deletion of exon 2/3 splice acceptor site. They showed strong expression of VEGF.II) Expression of angiogenic factor and tumor suppressor gene, and their clinicopathological implications in oral squamous cell carcinomasWe investigated the expression of VEGF, PD-ECGF, VEGF-C, p53 and p16, and their clinicopathological implications in oral squamous cell carcinomas by immunohistochemistry. Intratumor microvessel density was evaluated with the number of positive microvessels for CD31 antibody. Results : VEGF-C expression correlated with lymph node metastasis (p < 0.01). According to stepwise logistic regression analysis, univarate analysis showed lymph node metastasis correlated with mode of invasion, VEGF-C expression and vessel invasion (p < 0.05). Moreover, multivariate analysis revealed that mode of invasion and VEGF-C expression were the exclusive indipendent factors influencing lymph node metastasis (p < 0.05). PD-ECGF correlated with, intratumor microvessel density (p < 0.05). VEGF, tumor differentiation, mode of invasion and lymph node metastasis were found to be significant in survival probability (p < 0.05), and VEGF and lymph node metastasis were independent prognostic factors in the Cox's multivariate proportional hazard model. Conclusions : It was suggested that the expression of VEGF, PD-ECGF, and VEGF-C in oral squamous cell carcinoma mainly correlated with prognosis, angiogenesis, lymph node metastasis respectively. However, tumor suppressor gene p53 and p16 did not correlate with metastasis and prognosis.