Association between fibrinolysis and leukocyte integrin receptor Mac-1 in leukemia.

本研究旨在检测白血病细胞上Mac-1 （CD11b/CD18整合素粘附分子）的表达，以及白血病细胞同型同源物中纤溶酶活性和弹性酶-α 1蛋白酶抑制剂（E-α IPI）复合物的浓度。共检查了14例患者：11例急性白血病（AML 7, ALL 3， LGL白血病1）；1例骨髓增生异常综合征（RAEB-T）；2例慢性髓细胞白血病（CML）。流式细胞术检测白血病细胞的Mac-1， S2251显色法检测血浆溶酶活性，三明治免疫法检测E-α IPI复合物浓度。急性髓单核细胞白血病（AMMoL）患者的Mac-1表达高于其他白血病患者，尤其是急性早幼粒细胞白血病（APL）。另一方面，APL合并DIC患者的纤溶酶活性明显升高。APL、AMMoL或CML患者的E-E_0 IPI复合物水平均有显著至中度升高。E- a IPI复合物的浓度在DIC患者和非DIC患者之间无显著差异。从这些结果来看，提示白血病，特别是APL的高纤溶参与白细胞弹性酶而不是Mac-1。

The aim of this study was to assess the expression of Mac-1 (CD11b/CD18 integrin adhension molecules) on the leukemic cells, and to evaluate the plasmin activity in leukemic cell homoginates and the concentration of elastase-α 1-proteinaseinhibitor (E-α IPI) complex. A total of 14 patients were examined : 11 with acute leukemia (AML 7, ALL 3, LGL leukemia 1) ; 1 with myelodysplasia syndrome (RAEB-T) ; 2 with chronic myelocytic leukemia (CML).Mac-1 on leukemic cell was detected by flow cytometory, whereas plasmin activity was measured using chromogenic assay by S2251, and concentrations of E-α IPI complex were assayed using a sandwich-type immunoassay.Mac-1 expression was found to be higher in the patients with acute myelomonocytic leukemia (AMMoL) than other leukemic patients, especially acute promyelocytic leukemia (APL). On the other hand, plasmin activation markedly increased in APL patients with DIC. Marked to moderate elevation of E-E_0 IPI complex levels was observed in patients with APL, AMMoL or CML. The concentrations of E- a IPI complex did not show any significant differences between patients with DIC and patients without DIC.From these results, it is suggested that hyperfibrinolysis in leukemia, especially APL, participates in leukocyte elastase rather than Mac-1.