Assessment of biological effect of low dose butyltin concentrations using small crustaceans with short-life span

在许多被认为具有内分泌干扰作用的人造化学品中，由于对三丁基锡严重的生态毒理学影响的关注，大多数发达国家对在水产养殖和小型船只中使用三丁基锡实行了法律限制。小角甲壳类动物，活动能力弱，寿命不足3个月。此外，Caprella spp缺乏退化TBT（三丁基锡）的能力。这些特征表明Caprella是对TBT最敏感的海洋无脊椎动物之一。2001年对户内海西部及邻近海域丁胺素的野外调查表明，丁胺素的TBT含量在n.d（未检出）~ 39.1 ng TBT/L之间。目的：评价TBT对卡氏蝇的生物学效应。在胚胎发育过程中，暴露于0 ~ 10000 ng TBTCl/L的5种浓度下，在无污染海水中饲养1代以上。即使在10 ng TBTCl/L的浓度下，成熟期的成活率也不足50%。因此，2001年在濑户内海检测到的TBT浓度仍然影响卡佩拉氏菌的存活率。

Of many artificial chemicals which are though to have endocrine disruptor effects, the concern over the serious ecotoxicological impact of tribtyltin (TBT) led to legal restrictions on the usage of TBT in aquaculture and small boats in the majority of developed countries. Caprella spp.are small amphipod crustaceans with reduced movement and a life-span of less than 3 months. Moreover, Caprella spp.have lack the ability of degenerating TBT (tributyltin). These characteristics indicate that Caprella spp.are one of the most sensitive marine invertebrates to TBT. The field survey from the western part of Seto Inland Sea and adjacent areas on the butyltins during 2001 indicated that the TBT ranged N.D.(not detected) to 39.1 ng TBT/L. To assess the biological effect of TBT to Caprella., juveniles of Caprella danilevskii, which had been exposed to 5 concentrations from 0 to 10,000 ng TBTCl/L during embryonic development, reared in non-contaminated seawater more than 1 generation. The survival rate at the mature stage was less than 50 % even at 10 ng TBTCl/L. Thus, the TBT concentrations detected from the Seto Inland Sea during 2001 still influenced the survival rate of Caprella spp.