Construction of congenic strains from epileptic model rat NER

NER是一种癫痫动物模型，其表现出自发性强直-阵挛性惊厥，开始为颈部和前肢阵挛、疯狂跳跃/奔跑、强直性反张姿势，并发展为强直性，然后是阵挛性惊厥，随后是发作后弛缓。发作期皮层和海马脑电图的特征是高电压尖峰，随后是弥漫性尖峰和波或多尖峰和波复合体。癫痫发作对戊四氮、抛掷和经角膜电击敏感，但对触觉、光或声刺激或经耳电击不敏感。在CNS中未发现病理变化。本研究利用F344-NER 1 ^/ner 1 ^和NER.F344-ner 1 ^/ner 1 ^两个同基因系，通过对F344-NER 1 ^<NER>/ner 1 ^<NER>和NER.F344-NER 1 ^ /ner 1 ^两个同基因系的研究，发现NER 1 ^/ner 1 ^与NER 1 <F344>^ /ner 1 ^有相似的遗传关系，NER 1 ^/ner 1 ^与NER 1 ^ /ner 1 ^有相似的遗传关系<F344>。前者预计将显示GTC，而后者预计不会显示GTC。在今后的研究中，我们认为利用NER、F344以及这两个同源株系进行严格的癫痫动物实验是可能的。

NER is an animal model of epilepsy that exhibit spontaneous tonic-clonic convulsions, beginning with neck and forelimb clonus, wild jumping/running, opisthotonic posturing, and evolving to tonic, then clonic convulsion, followed by postictal flaccidity. Ictal cortical and hippocampal EEGs are characterized by high-voltage spikes followed by diffuse spike-and-wave or polyspike-and-wave complexes. Seizure susceptibility to pentylenetetrazol, tossing, and transcorneal electroshock, but not to tactile, photic, or acoustic stimuli, or to transauricular electroshock. No pathologic changes were demonstrated in the CNS. Spontaneous convulsions are controlled by a major autosomal recessive gene for epilepsy, that are comparable to generalized tonic-clonic seizures in humansIn this project, we have generated two congenic lines of F344.NER-ner1^<NER>/ner1^<NER> and NER.F344-ner1^<F344>/ner1^<F344>. The former is expected to to show GTC, and the latter is expected not to show GTC. In the future, it is considered that strict animal experiment for reseach on epilepsy will be possible using NER, F344 and these 2 lines of congenic strains.