Relationship between mucosal immune response in suncus tonsil and onset of focal infection

本研究于2000 ~ 2001年从免疫组织学和免疫病理学的角度探讨扁桃体腭突粘膜免疫反应与扁桃体局灶性感染发病的关系。试验的分离和纯化IgA级分从提取物的胃肠道的suncus是不完整的，然而，免疫组织化学染色的扁桃体清楚地表明，该组织揭示了较高的活性，在该网站的扁桃体隐窝上皮的伊加生产，而不是IgG或IgM的生产。用粘膜免疫佐剂免疫扁桃体，可在免疫的扁桃体及其局部淋巴结内诱导特异性抗体的形成。有趣的是，一些扁桃体反应动物在其肾小球中具有节段性或膜性模式的抗体特异性沉积物，但它们没有显示出任何系膜增殖或新月体形成的迹象。虽然扁桃体伊加的产生与肾小球肾炎之间没有直接的证据，但所获得的结果表明，免疫复合物在病理性肾小球中的沉积可能是由来自抗原刺激的扁桃体的循环伊加诱导的，表明器官不仅作为局部免疫应答的诱导部位而且作为效应部位起重要作用。此外，还提示悬雍垂是研究扁桃体局灶性感染发病机制的有用实验动物。

This study was performed to investigate the relationship between mucosal immune response in suncus palatine tonsil and the onset of tonsillar focal infection at the immunohistological and immunopathological points of view, in 2000 to 2001.The tonsils showed typical structures as mucosa-associated iymphoid tissues at the level of light and electron microscopy. Trial for isolation and purification of IgA-fraction from the extract of gastrointestinal tracts of suncus was incomplete, however, the immunohistochemical staining of the tonsils clearly showed that the tissues revealed a higher activity with regard to IgA production at the site of the tonsillar crypt epithelium, rather than IgG or IgM production. Immunization of antigen with mucosal immune adjuvant to the tonsils induced specific antibody formation within the immuned tonsils and their regional lymph nodes. Interestingly, some tonsillar responder animals had antibody-specific deposits in their glomerurus with segmental or membranous patterns, but they did not show any sign of mesangial proliferation or crescerlt formation. Although there is no direct evidence between tonsillar IgA production and the glomerulonephritis, the obtained results suggest that the deposition of the immune complex in pathological glomerulus may be induced by the circulating IgA derived from the antigen-stimulated tonsils, showing the organs play an important role as not only inductive site but also effector site of local immune response. Moreover, it is suggested that the suncus is useful experimental animal for research of the onset mechanism of tonsillar focal infection.