Molecular devices by functionalization of modified DNA

本研究的目的是将共价修饰的DNA与不同的功能发色团结合起来，赋予DNA各种功能，从而实现对DNA的调控，明确修饰后的DNA在生物体系统中作为分子器件的可能性。特别是，为了阐明DNA中的空穴转移与DNA分子线的构建有关，我们在纳米尺度上研究了具有光功能发色团的修饰DNA的光功能，依赖于核碱基序列。为了阐明纳米尺度和纳米皮秒尺度上的电荷分离、空穴迁移和电荷复合，在一个DNA上不仅修饰了一个生色团，而且还修饰了几个生色团。通过分子组装手段精确操作功能分子的技术是自下而上型纳米技术的必要条件，需要建立根据人工信息进行分子组装的方法。DNA是最强大的分子，它可以与多种分子实现精确的分子组装，并具有通过DNA的化学修饰可以引入各种功能分子的优点。因此，我们合成了具有分枝结构的DNA单元，构建了基于自组装的规则的刚性DNA结构，并成功地构建了DNA纳米结构，从而构建了一座桥接DNA。为了更准确地通过修饰DNA的功能化来建立分子器件，实现叶绿素、光功能和复杂功能的多功能是非常重要的。还有必要阐明构建具有高性能和分子器件功能的修饰DNA的方法，以及开发修饰DNA作为活体系统分子器件的可能性。

In this research, it is aimed that various functionalities are given to modification DNA by using DNA modified covalently with various functional chromophores, controlling the functionalities can be established, and the possibility of modified DNA as molecular devices in the living body system is clarified. Especially, in order to clarify the hole transfer in DNA with respect to the construction of DNA molecular wire, photofunctions of modified DNA having photofunctional chromophores has been studied in the nano meter scale depending on the nuclear base sequences. Not only one chromophore but also several chromophores are modified on one DNA in order to clarify the charge separation, hole migration, and charge recombinataion in the nano meter scale and in the nano-pico second time scale. Technology that accurately operates functional molecule by means of molecular assembly is necessary to the nanotechnology of a bottom-up type, and it is necessary to establish the method of molecular assembly according to artificial information. DNA is the most powerful molecule where an accurate molecular assembly is achieved with many kinds of molecules and has the advantage that a variety of functional molecules can be introduced by the chemical modification in DNA. Therefore, DNA unit with the branch dividing structure was synthesized to construct a regular and rigid DNA structures based on self-assembly, and we succeeded to construct DNA nano structure to use this constructing a bridge DNA. In order to establish molecular devices by functionalization of modified DNA more precisely, to achieve the multifunction like the chlorophyll, photofunction, and complex functions is very important. It is also necessary to clarify the method of construction of modified DNA with a high performance and molecular device as a biomolecular function, and to develop the possibility of modified DNA as molecular devices in the living body system.