ARTIFICIAL EXTRACELLULAR MATRIX MADE OF ALGINATE

在本研究中，我们旨在模拟多细胞生物细胞外基质的分子结构和功能，开发一种用于组织和器官修复的人工细胞外基质。海藻酸盐人工细胞外基质的骨修复是一种基于BMP-2结合细胞表面受体结构设计的新型骨形成肽（BFP），可提高骨前体细胞ALP活性，增加骨钙素的表达。abfp -海藻酸盐凝胶偶联物植入大鼠小腿肌后可诱导异位骨形成，并在植入9周后对长度为25mm的兔尺骨缺损有加速骨修复的作用。肝素/海藻酸盐人工细胞外基质中肝素结合生长因子的缓释研究表明，一种新型肝素/海藻酸盐人工细胞外基质可持续释放bFGF、HGF、VEGF等肝素结合生长因子。在动物实验中，这种材料增加了血管、皮肤、神经和肾脏的空气。细胞死亡控制材料基于死亡配体家族、TNFα FasL和TRAIL的结构同源性，我们创造了新的细胞死亡抑制肽。tnf α-抑制肽*对海马神经干细胞的存活和向神经元细胞的分化具有显著的促进作用。该肽还显示骨髓基质细胞向神经元细胞分化。将硅醇基引入海藻酸盐中，采用仿生工艺制备了三维磷灰石-海藻酸盐复合物。该复合材料可加速大鼠胫骨缺损的修复。结论海藻酸盐人工细胞外混合物具有细胞支架的作用，具有稳定和释放生长因子，控制细胞生长、分化和死亡的作用。基于藻酸盐人工细胞外基质的这些功能，我们开发了用于骨、神经、皮肤、血管和肾脏修复的有用材料。进一步的研究将使藻酸盐人工细胞外基质为组织工程和再生医学提供有用的材料。少

In this research, we aimed to develop an artificial extracellular matrix for tissue and organ repair, mimicking the molecular structure and functions of the extracellular matrix in multi cellular organisms.1.Bone repair by the alginate artificial extracellular matrixA novel bone-forming peptide (BFP) designed based on the structure of BMP-2 bound to cell surface receptor, increased ALP activity, and increased expression of osteocalcin of bone precursor cells. ABFP-alginate gel conjugate induced ectopic bone formation when it was implanted into the rat calf muscle, and also showed accelerated bone repair in the rabbit ulnar defects with 25mm length after 9-weeks implantation.2.Slow release of heparin-binding growth factors from heparin/alginate artificial extracellular matrixWe showed sustained release of the heparin-binding growth factors, such as bFGF, HGF, VEGF, and so on, from a novel heparin/alginate artificial extracellular matrix. In animal experiments, the material increased rep … More air of blood vessels, skins, nerves, and kidney.3.Cell-death controlling materialWe create novel cell death-inhibiting peptides based on the structural homology of the death ligand family, TNFα FasL, and TRAIL. The TNFα-inhibiting peptide *owed marked cell survival and differentiation of hippocampal neural stem cells to neuronal cells. The peptide showed also differentiation of bone marrow stromal cells to neuronal cells.4.Apatite-alginate gel 3D-hybridIntroduction of silanol groups into alginate gave 3D apatite-alginate hybrid using biomimetic process. This hybrid material accelerated repair of rat tibial bone defects.ConclusionThe alginate artificial extracellular mix revealed to act as a cell scaffold, to stabilize and release of growth factors, and to control cell growth, differentiation and death. Based on these functions of the alginate artificial extracellular matrix we developed useful materials for the repair of bone, nerve, skin, blood vessels, and kidney. Further investigation will make the alginate artificial extracellular matrix to make useful materials for tissue engineering and regenerative medicine. Less