Research for inorganic salts enhancement on bio-activity of plant growth registers and its practical use for crop production

种植者已经知道，添加一种无机盐作为添加剂可以增强除草剂的活性。然而，关于这一现象的生理机制的研究却很少。本研究着重从生理上解释盐渍增强的生理机理，利用一些生物测定系统模拟PGR活性，并尝试将该技术应用于实际作物生产。本文报道了10mm -硝酸铵（AN）显著增加了NAA诱导的生长素（2,4- d）的产生，AVG抑制了1μL NAA模拟喷雾处理豇豆（Vigna unguiculata L.）叶片乙烯的产生。氮素还能增强ga3诱导的矮水稻（Oryza sativa L., cv.）叶鞘伸长。Tan-ginbozu)。低湿和高湿条件下，微滴干燥后的AN增强效应最明显，而持续高湿条件下AN增强效应最小或不显著。硝酸、硫酸和氯化物铵盐对NAA诱导豇豆叶盘乙烯产率的影响最大，而钾、钠和钙等硝酸盐对NAA诱导豇豆叶盘乙烯产率无影响。在pH为6时，当NAA分子以游离阴离子的形式存在时，AN对NAA增强乙烯生成的影响最为明显，但在pH为3时，这种影响不显著。NAA处理后的离体AN预处理对乙烯诱导也有效果。我们可以说明一类无机盐提高了某些植物生长调节剂的生物活性，并提出了一种假设，即无机盐使角质层脂质聚合物的密度降低，从而刺激角质层膜的大量通透性。在实际果园中，在GA3中添加a对GA成花的抑制作用较强。这项技术有助于减少对环境有害的农用化学品和照明。

It has been known in growers that the addition of a kind of inorganic salts as additives enhanced the activity of herbicide. However few studies have not be conducted on the physiological mechanism over the phenomenon. This study is focusing on the physiological explanation of the salt enhancement on simulation of PGR activity with some bioassay systems and attempting this technique for practical crop production.We now report that 10mM-ammonium nitrate (AN) significantly increased auxin (NAA, 2,4-D) induced and AVG inhibited ethytene production by cowpea (Vigna unguiculata L.) leaf disks treated with simulated spray dropfets (1μL) of NAA. AN also enhanced GA3-induced elongation of the leaf sheath of dwarf rice (Oryza sativa L., cv. Tan-ginbozu).The AN enhancement eflect was most pronounced under low humidity and high humidity following dryness of droplet under tow humidity, while it was minimal or not significant at continual high humidity. The ammonium salts of nitrate, sufiate and chloride were most active while the nitrate salts of potassium, sodium and calcium were without effect on NAA induction of ethylene production in cowpea leaf disks. The effect of AN on NAA-enhanced ethyfene production was most pronounced at pH 6, when the NAA molecule was present as a dissociated anion, though that effect was not significant at pH 3. The isolated AN preteeatinent followed NAA treatment was also effective on ethylene induction.We can represent that a kind of inorganic salts enhanced bio-activity of some plant growth regulators, and can suggest the hypothesis that the sate loosen the denseness of cutidar lipoidal high polymer to stimulate the substantial permeability of cuticlar membrane.In the practical orchards, AN application as additive in GA3 inhibited stronger the flower formation by GA. This technique can contribute to reduction of agrochemicals and lightening bad for environment.