Studies on the method for searching endocrin disrupting chemicals and the regulation of accumulation of the chemicals in the body of animals

这项研究是为了寻找干扰内分泌的化学物质，使用雄性鸡或培养的雄性鸡的肝细胞。以卵黄原蛋白mRNA表达量为指标。卵黄原蛋白是母鸡肝脏中表达的蛋黄蛋白。具有雌激素样作用的化学物质可诱导雄性鸡肝脏中卵黄蛋白原的表达。采用rt-PCR法检测Vittellogenin mRNA。使用这种方法，我们发现了一种杀虫剂的类似雌激素的作用（化学物质的名称没有显示）。．此外，我们还以雄鸡为研究对象，调查了霞浦县水中内分泌干扰物的污染情况。饲喂湖水的雄鸡肝脏中未检测到卵黄原蛋白mRNA的表达。采用肝细胞原代培养法建立肝功能实验模型。本研究采用非血清培养基培养肝细胞，寻找干扰内分泌素的化学物质。在有或无雌激素的培养肝细胞中检测卵黄原蛋白mRNA的表达。这个噪音可以在这个项目期限内消除。

This study was conducted in an attempt to search endocrin disrupting chemicals, using male chickens or cultured hepatocytes of male chickens. The vitellogenin mRNA expression was selected as an index. Vitellogenin is the egg yolk protein expressed in the liver of female chickens. The chemicals with estrogen-like effect induce the vitellogenin expression in the liver of male chickens. Vittellogenin mRNA was detected using the rt-PCR methods.Using this method, we found the estrogen-like effect of an insecticide (Name of the chemical is not shown. ).Furthermore, we investigeted the pollution by endocrin disrupting chemicals of the water of Lke Kasumigaura, using male chickens. The vitellogenin mRNA expression could not be detected in the liver of male chickens administered the lake water.A primary culture of hepatocytes was applied to experimental models of liver function. In this study, cultured hepatocytes with non-serum medium were used to search endcrin disrupting chemicals. Vitellogenin mRNA expression was detected in cultured hepatocytes with or without estrogen. This noize could be eliminate within this project term.