Molecular mechanisms underlying the rearrangement of intermediate filaments networks.

在这项研究中，我发现plectin结合了一种新的中间丝蛋白Desmuslin，并由此从大鼠骨骼肌cDNA文库中克隆了Desmuslin基因。该蛋白与结蛋白和粘附素共同定位于Z盘周和肌膜下。Desmuslin可能在中间丝网络的重排中起关键作用。我研究了中间丝(IF)蛋白在大鼠骨骼肌纤维发育过程中定位的空间和时间序列，结蛋白和plectin。在未成熟肌管中，结蛋白IF平行于肌管纵轴排列。后来，结蛋白IF横向定向，并与Z型盘对齐，就像在成人肌肉纤维中发现的那样。Plectin在肌管成熟过程中表现出类似的重排模式，但其重排先于结蛋白IF。

In this study I have found that plectin binds a novel intermediate filament protein, desmuslin, and thus cloned desmuslin cDNA from rat skeletal muscle cDNA library. This protein was colocalized with desmin and plectin around Z-discs and beneath the sarcolemma. Desmuslin may play a pivotal role in the rearrangement of intermediate filament networks.I have examined spatial and temporal sequences of localization of intermediate filament (IF) proteins, desmin and plectin, during the development of rat skeletal muscle fibers. In immature myotubes, desmin IFs were arranged parallel to the longitudinal axis of myotubes. Later desmin IFs oriented transversely and were aligned to Z-discs, as found in adult muscle fibers. Plectin showed similar rearrangement pattern during the maturation of myotubes, but its rearrangement preceded that of desmin IFs.