EFFECT OF HIGH NaCl DIET ON HEPATIC Na^+ RESEPTOR SENSITIVITY AND EXPRESSION OF NKCC1 IN RATS

· We previously reported that the bumetanide-sensitive Na^+-K ^+-2 Cl ^- cotransporter （NKCC1） is involved in the hepatic Na^+ and K^+ sensor mechanism。在目前的研究中，我们研究了高NaCl或高KCl饮食对Hepatic Na^+和K^+受体敏感性和NKCC 1表达的影响。RT-PCR和Western blots已被用来衡量NKCC 1 mRNA和蛋白质表达，尊重。在Na^+或K^+合并依赖性指示器中输入高水平NaCl或同位素KCl + NaCl解决方案的门户肠道增加了组效应神经活动（HANA）。经过4周的高NaCl或高KCl饮食， HANA的反应被认为与动物喂养正常饮食相比，NKCC 1的表达被减少了。这些结果显示了一个高NaCl或高KCl饮食中NKCC 1表达在肝脏中的表达，它导致了对Hepatic Na^+-和K^+-受体敏感性的降低。这是众所周知的，因为它是一种超声波的内部半导体注射。 ... More 氯化钠素在动脉压力中增加，通过激活对称性神经系统和血管紧张素。However，这种反应的中央路径并不清楚，因为通过合理空间和临时解决方案检测到激活的脑区域中的氯化钠的扩散是不清楚的。Recently， we have reported the usefulness of Mn^<2+> contrasted magnetic resonance imaging （MRI） in hypothalamic imaging。在这一方法中，随着行动潜力的触发， Ma^<2+>吃者通过Ca^<2+>途径获得内部空间。因此，激活的神经元细胞含有更多的Mn^<2+>，在T_1加权MRI中增加信号强度的结果是什么？在目前的研究中，中央激活对高钠氯化钠（1.5 M， 10微升）的内部和后期静脉注射作出反应，已在非正式大鼠中检测到使用这一技术。周期性区域是第一个活跃和活跃的区域，被转移到大脑辅助区。“中央激活的时间课程是可以比较的，可以与局部压力和对称神经激活响应的时间课程。”Less（低）

・ We previously reported that the bumetanide-sensitive Na^+-K^+-2Cl^- cotransporter (NKCC1) is involved in the hepatic Na^+ and K^+ sensor mechanism. In the present study, we examined the effects of a high NaCl or high KCl diet on hepatic Na^+ and K^+ receptor sensitivity and NKCC1 expression in the liver of Sprague-Dawley rats. RT-PCR and Western blots were used to measure NKCC1 mRNA and protein expression, respectively. Infusion of hypertonio NaCl or isotonic KCl + NaCl solutions into the portal vein increased hepatic afferent nerve activity (HANA) in a Na^+ or K^+ concentration-dependent manner. After 4 weeks on a high NaCl or high KCl diet, HANA responses were attenuated compared to in animals fed a normal diet, and NKCC1 expression was reduced. These results show that a high NaCl or high KCl diet decreases NKCC1 expression in the liver and it causes a reduction in hepatic Na^+- and K^+-receptor sensitivity.・ It is well known that an intra-cerebroventricular injection of hypertonio … More NaCl elicits an increase in arterial pressure through activation of sympathetic nervous system and vasopressin. However, the central pathway of this response is not clear, because of diffusion of injected NaCl throughout the brain and a difficulty in detecting activated brain regions with reasonable spatial and temporal resolutions. Recently, we have reported the usefulness of Mn^<2+> contrasted magnetic resonance imaging (MRI) in hypothalamic imaging. In this method, following the triggering of action potential, Ma^<2+> eaters the intracellular space through Ca^<2+> pathways. Thus, activated neuronal cells contain more Mn^<2+>, which results in an increased signal intensity in T_1-weighted MRI. In the present study, the central activation in response to an intra-lateral ventricular injection of hypertonic NaCl (1.5 M, 10 μl) was detected using this technique in anesthetized rats. The peri-ventricular region was first activated, and activated regions moved into the brain parenchyma. The time course of the central activation was comparable to time course of arterial pressure and sympathetic nerve activity responses. Less