Molecular pathology of tumor cell origin and the oncogenesis in renal angiomyolipoma

肾血管平滑肌脂肪瘤的血管成分按组织学类型分为肌动脉、小肌动脉、肌静脉、毛细血管和鹿角型/窦状型血管。同心向心型增殖的平滑肌细胞的组织病理学分析揭示了增殖的平滑肌细胞对聚集的增殖动脉的侵袭行为。我们建立了日本女性两种不同大小的X-连锁HUMARA基因的PCDR产物的长度和数量的分析系统。我们选择性地从肾血管肌脂肪瘤的不同成分中取样，利用“LM200奥林巴斯显微解剖显微镜”，扩增HUMARA基因，HUMRA分析显示正常血管的平滑肌细胞为多克隆，显示其非肿瘤性；另一方面，增殖中的平滑肌细胞HUMARA基因扩增产物单峰消失或明显减少，显示其单克隆性。我们目前正准备调查其他尚未确定为肿瘤性或非肿瘤性的增殖性病变，以及肾血管肌脂肪瘤的其他成分。

The different types of the vascular component of the renal angiomyolipoma were classified histologically into muscular artery, small muscular artery, muscular vein, capillary and staghorn type / sinusoid type vessels.The histopathologic analysis of proliferative smooth muscle cells in concentric proliferative pattern revealed the invasive behavior of the proliferative smooth muscle cells to gathered proliferative arteries.We established the analysis system of the fragment length and the quantity of the PCDR product in two different-sized X-linked HUMARA gene in Japanese women.We sampled the different component of the renal angiomyolipoma selectively, utilizing the "LM 200 Olympus microdissection microscope", amplified the HUMARA gene, and analyzed them to determine the neoplastic tendency of the component cells.The smooth muscle cells of normal appearing vessels were polyclonal by HUMARA analysis, demonstrating their non-neoplastic nature.On the other hand, the proliferating smooth muscle cells showed disappearance or significant decrease of the amount of one peak of HUMARA gene amplification product, indicating their monoclonal nature.We are now preparing to investigate other proliferative lesions that have not been decided neoplastic or non-neoplastic in nature, as well as other components of the renal angiomyolipoma.