Distribution of ABO blood group H type 1-4 sugar chain antigens in human tissue from secretor or non-secretor individuals

1.应用免疫组化技术阐明了H 1型、H 2型和H 3/4型抗原在人体组织中的分布。在肺中，分泌型支气管腺的粘液细胞被H 1型抗体染色，并且支气管粘膜表面的尖端被H 1型和H 3/4型强烈染色。但两种抗体均不染色非分泌型。支气管上皮基底细胞无论分泌型或非分泌型均为H 2型抗体染色。来源于分泌型和非分泌型的肾远曲小管分别被H 2型和H 3/4型染色。来源于分泌器的集合管主要被H 1型抗体染色，而来源于非分泌器的集合管染色不明显。2.北方杂交和原位杂交技术检测FUT 1和FUT 2基因在mRNA水平的表达。我们使用腺癌细胞系（MCAS、PC-3、CCK-81、CoCM-1、WiDr和SW 837）和表皮样癌细胞系（HeLa和HEp-2）。FUT 1在所有人癌细胞系中表达，FUT 2在除HeLa、HEp-2和PC-3之外的所有细胞中表达。结果显示，两种FUTs在癌细胞中均表达，而在表皮样癌细胞中仅FUT 1 mRNA表达的趋势。此外，从横结肠到直肠（从CCK-81到SW 837，见上文），腺癌细胞系中FUT 1 mRNA的表达量增加。我们将岩藻糖基转移酶在各种癌组织中的表达数据与我们的研究结果进行了比较，发现岩藻糖基转移酶在癌组织中的异常表达受mRNA水平的调节。

1.The distribution of H type1, H type2, and H type 3/4 antigens in human tissue were clarified by use of immunohistochemical techniques. In lungs, mucous cells of a bronchus gland of secretor type was stained by H type1 antibody, and the tip of the bronchus mucous membrane surface was by H type1 and H type3/4 strongly. However, neither of antibodies stained that of non-secretor. The basal cells of bronchus epidermis were stained by H type2 antibody regardless of secretor or non-secretor. The distal convoluted tubule of kidney derived from secretor or non-secretor was stained by H type2 and H type3/4. The collecting duct derived from secretor was stained by H type1 antibody mainly, and it was not stained by that from non-secretor very much. In addition, only the collecting duct from secretor was stained by H type3/4 antibody.2.With Northern blot and in situ hybridization analysis, we demonstrated expressions of FUT1 and FUT2 gene at the level of mRNA. We used adenocarcinoma cell lines (MCAS,PC-3,CCK-81,CoCM-1,WiDr, and SW837) and epidermoid cancer cell lines (HeLa and HEp-2). FUT1 was expressed in all of human carcinoma cell lines, and FUT 2 was expressed in all of cells except HeLa,HEp-2, and PC-3. As a result, the tendency that both of FUTs were expressed in the carcinoma cells, and only FUT1 mRNA was expressed in the epidermoid cancer cells. In addition, the quantity of expressions of FUT1 mRNA increased in adenocarcinoma cell lines from a transverse colon to the rectum (from CCK-81 to SW837, see above). We compared the data have been reported about expression of the fucosyltransferase in various cancer tissue with the result of our research, and found that aberrant expressions of fucosyltransferase in the cancer tissue were regulated by a level of mRNA.