The newly born baby/infant type pepsinogen It's regulation mechanism and the potential of Onco-protein

1)基于我们克隆的新生婴儿/婴儿型胃蛋白酶原(PGF)和凝乳酶(CHY)的蛋白质一级结构的结果，我们制备了合成肽免疫兔子，获得了两种针对PGF的抗体。免疫组织化学显示，PGF出现在大鼠胚胎的第16天，产生的细胞将至少部分地构成未来的胃腺。2)我们一直在用我们克隆的PGF基因文库进行这些基因的基因组克隆。到目前为止，我们已经检测了41个阳性克隆，但都是PGC的克隆，我们还没有获得PGF的基因组克隆。3)我们用我们开发的胃肠上皮分离方法从大鼠胎胃粘膜上皮组织中提取了RNA和蛋白质的可溶性部分。在胚胎后期，从胃的形态形成期到出生，我们研究了…的表达。在基因水平(定量聚合酶链式反应)和蛋白水平(酶谱分析)上，PGF CHY和成人型胃蛋白酶原(PGA)的表达水平较高。结果表明，胎胃在第15天出现PGF基因的表达，随后出现CHY基因的表达。PGF基因的表达主要见于胃形态形成期，并可见少量PGA表达。在出生前，CHY基因表达上调超过PGF的表达，在断奶期间，PGA的表达超过CHY的表达。4)在新生大鼠的体内实验系统中，我们发现通过对大鼠胃粘膜施加分化诱导因子，如肾上腺皮质类固醇，胃蛋白酶原基因的表达从PGF转换为PGA。利用我们建立的胚胎胃上皮细胞原代培养体系，我们证实了通过给予类固醇和二丁基-cAMP等诱导分化因子，基因的表达从PGF或CHY转变为PGA。较少

1)Based on the results of the protein primary structures of the newly born baby/infant type pepsinogen (PGF) and chymosin (CHY) identified by our cDNA cloning, synthetic peptides were prepared to immunize rabbits and 2 kinds of antibodies were obtained against PGF Immunohistochemistry revealed that PGF appeared on Day 16 of rat fetuses and the producing cells would, at least in part, constitute a gastric gland in the future.2)We have been performing genomic cloning of these genes with the rat genomic library previously established using the PGF cDNA we cloned. To date, we have examined 41 positive clones, but all of them were clones for PGC and we have not obtained genomic clones for PGF.3)We extracted RNA and protein soluble fractions from gastric mucosal epithelial tissue of rat fetuses using the separation method of the gastrointestinal epithelium that we developed. In the later fetal period, from the morphology formation period of the stomach to birth, we investigated the expressio … More n of PGF CHY, and adult type pepsinogen (PGA) at the gene levels (by quantitative PCR) and protein levels (by zymography). As a result, the PGF gene expression first appeared on Day 15 in the fetal stomach, and later the expression of the CHY gene appeared. The expression of the PGF gene was mainly observed in the period of morphological formation of the stomach and a trace of PGA expression could be found. Just before birth, the CHY gene was upregulated to exceed the expression of PGF, and in the weaning period, in turn, the PGA expression exceeded the CHY expression.4)Using the in vivo experiment system on newly born rats, we found that the expression of the pepsinogen genes switched from PGF to PGA by stimulating the rats with differentiation-inducing factors on the gastric mucosa, such as adrenal corticosteroids. Using the primary culture system of fetal gastric epithelium that we developed, we confirmed that the gene expression switched from PGF or CHY to PGA by administering differentiation-inducing factors on gastric epithelial cells, such as steroids and dibutyl-cAMP. Less