Therapeutic trial of prion infected mice by continuous infusion of anti-prion agent to brain

1)建立朊病毒感染细胞系，评价抗朊病毒药物的体外效果。2)筛选可能具有抗朊病毒作用的化学制剂和现有药物。3)聚硫酸戊聚糖（PPS）的抗朊病毒效果最好。4)为了提高脑输送率，我们尝试通过化学反应对PPS进行修饰。5) Morphorino不能降低体外PrPc的生成。6)我们建立了朊病毒病患者使用PPS和Quinacrine的临床方案。

1) We established prion-infected cell line to evaluate in-vitro effect of anti-prion agents.2) We screened chemical agents and already existing medicines which may have possible anti-prion effect.3) Pentosan Polysulfate (PPS) showed maximum anti-prion effect among the agents we tested.4) To acquirer increased ratio of brain delivery, we are try to modifying PPS by chemical reactions.5) Morphorino failed to reduce PrPc production in vitro.6) We established the clinical protocol of patients with Prion disease using PPS and Quinacrine.