Microbicidal efficacy ozonated water against Candida albicans adhered to acrylic denture plate

<Objectives>众所周知，臭氧是一种强大的抗细菌、真菌和病毒的抗菌剂。我们研究了臭氧水对。丙烯酸义齿板上的白色念珠菌。<Methods>用C.白色念珠菌用无菌盐水洗涤并浸入臭氧水（4 mg/L）中60分钟后，计数附着的白色念珠菌的数量。在一些实验中，用臭氧化水结合超声处理来处理测试样品。<Results>在暴露于流动的臭氧水（2或4 mg/L）1分钟后，几乎不存在活的白色念珠菌细胞。臭氧水和超声波处理的组合对粘附在丙烯酸树脂板上的白色念珠菌的活力有很强的影响。有没有显着差异，对白色念珠菌的抗菌活性之间的板浸泡在臭氧水与超声波处理和那些处理与市售义齿清洁剂。此外，电子显微镜分析显示，在暴露于流动的臭氧化水或浸入臭氧化水中超声处理后，少量的白色念珠菌保留在板上。<Conclusion>我们的研究结果表明，臭氧水的应用可能有助于减少义齿板上的白色念珠菌数量。

<Objectives> Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. We examined the effect of ozonated water against. Candida albicans on acrylic denture plate. <Methods> The heat-cured acrylic resins were cultured with C. albicans. Following washing with sterile saline and immersion in ozonated water (4 mg/L) for 60 minutes, numbers of the attached C.albicans was counted. In some experiments, the test samples were treated with ozonated water in combination with ultrasonication. <Results> After exposure to flowing ozonated water (2 or 4 mg/L) for 1 minute, viable C.albicans cells were nearly nonexistent. The combination of ozonated water and ultrasonication had a strong effect on the viability of C.albicans adhered to the acrylic resin plates. There were no significant differences in antimicrobial activity against C.albicans between plates immersed in ozonated water with ultrasonication and those treated with commercially available denture cleaners. In addition, electron microscopic analysis revealed that small amounts of C.albicans were remained on the plate, after exposure to flowing ozonated water or immersion in ozonated water with ultrasonication. <Conclusion> Our results suggested that application of ozonated water may be useful for reducing the number of C.albicans on the denture plates.