Study on pathogenesis of invebeterate oral mucous membrane diseases : Investigation of superoxide anion and various cytokine.

本研究的目的是通过研究双头孢菌素(BA)对临床症状的影响与细胞因子或抗氧化酶的免疫组织化学染色之间的关系，来探讨各种口腔膜疾病，特别是口腔扁平苔藓的发病机制。取30例口腔扁平苔藓患者的活检组织标本。免疫组织化学染色采用链霉亲和素-生物素法。用x^2检验和Logistic回归分析细胞因子(肿瘤坏死因子-α、干扰素-γ、白介素1-β)和抗氧化酶(含锰超氧化物歧化酶、谷胱甘肽过氧化物酶)的表达与临床疗效的相关性。研究结果如下：1.结果：1.肿瘤坏死因子-α、干扰素-γ和IL-1β的表达分别为56.7%(17/30)、73.3%(221/30)和43.3%(13/30)。肿瘤坏死因子-α的表达与干扰素-γ或IL-1的β表达呈显著正相关。肿瘤坏死因子α的负值与BA的作用呈统计学相关(P=0.046)。其中16/30例(53.3%)和17/30例(56.7%)检测到Mn-SOD和GPX的表达。提示某些细胞因子和抗氧化酶可能参与了口腔扁平苔藓的发病机制和口腔扁平苔藓的作用。

The purpose of this study was to investigate the pathogenesis of various oral membrane diseases, in particular oral lichen planus, by examining the relationships between effects of biscoclaurine alkariods (BA; cepharanthin^<(R)>) on clinical symptoms and imunohistichemical stainings of cytokines or antioxidant enzymes. 30 tissue samples were obtained by biopsy from the patients with oral lichen planus. Immunostaining was performed using the streptavidin-biotin method. Correlation between Clinical improvement by treatment of biscoclaurine alkaloids and expressions of cytokines (TNF-α, IFN-γ, IL-1β) or antioxidant enzymes (manganese-containing superoxide dismutase : Mn-SOD, glutathione peroxidase : GPX) were evaluated using x^2-test and logistic regression. The results were as follow.1. The expressions of TNF-α, IFN-γ and IL-1β were detected in 17/30 (56.7%), 221/30 (73.3%) and 13/30 (43.3%) of the cases.2. TNF-α expression had significant relationship between IFN-γ or IL-1β expression.3. The negativity of TNF a was statistically correlated with effects of BA (P=0.046).4. The expressions of Mn-SOD and GPX were detected in 16/30 (53.3%) and 17/30 (56.7%) of the cases.5. The expression of Mn-SOD was a significant factor for the effects of BA on clinical symptoms.These results suggest that some kinds of cytokines and antioxidant enzymes may be related to pathogenesis of OLP and action of BA on OLP.