Research on high-degree utilization of genetic information on aquaculture fish

我们制作了一个标准的比目鱼家族，用于遗传连锁图谱。与已报道的包含111个微卫星标记的日本比目鱼连锁图谱(Coimbra et al.，2003)相连接，新的连锁图谱包含400个微卫星标记、144个来自EST、MHC基因和5SrRNA基因的SNP标记。在本研究的结果中，MHC基因和5SrRNA基因分别位于第13和第23连锁群上。对来自北海道、岩手鱼、秋田鱼、山形鱼、新泻鱼、神奈川鱼和石川鱼的40条鱼进行了测试，结果表明，所有的鱼都有2n=48个基因，包括标准的遗传连锁图谱。我们对斑马鱼和河豚的同源性进行了部分比较，并发现了牙鲆的一些基因在这两种鱼中都是保守的，但位置比较复杂。为了比较日本比目鱼与河豚和/或斑马鱼的同源性，我们要求进一步开发锚定基因。此外，我们利用遗传连锁图确定了控制牙鲆对淋巴囊肿病抗性的一个主效遗传位点。

We produced a standard family of Japanese flounder, Paralichthys olivaceus, for genetic linkage maps. Connecting to a previously reported Japanese flounder linkage map with 111 microsatellite markers (Coimbra et al.,2003), a new linkage map was covered with 400 microsatellite markers, 144 SNP markers originated from EST, MHC gene and 5SrRNA gene. At the results of this study, MHC gene and 5SrRNA gene were located on Linkage group 13 and 23, respectively.Numbers of chromosomes in Japanese flounder have different opinions. We tested 40 fish originated from Hokkaido, Iwate, Akita, Yamagata, Niigata, Kanagawa and Ishikawa, and showed that all the fish have 2n=48 including the standard family for genetic linkage maps.Synteny to zebra fish and fugu fish was partially compared and some genes of Japanese flounder were conserved in both fishes, although the position made complexity.In order to compare the synteny between Japanese flounder and fugu fish and/or zebra fish, it is requested that anchor genes should be developed more.In addition, we identified a major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder, using the genetic linkage map.