Restoration of renal microcirculation by eNOS gene transfection for overcoming the antibody-mediated rejection and achievement of long-term renal allograft survival

1.通过对活体供体ABO血型不合肾移植抗体介导的排斥反应和已故供体肾移植缺血再灌注损伤的临床病理观察，分析和评价微循环损伤和微血管内皮细胞损伤的过程。建立GFP(+)转(-)大鼠骨髓移植模型，评价骨髓来源内皮祖细胞在肾缺血再灌注损伤损伤微血管细胞再生中的作用。我们发现，在肾脏缺血再灌注损伤中，骨髓来源的内皮细胞迁移并修复了受损的内皮细胞，这不仅得到了静态免疫病理方法的证实，也得到了实时相差显微镜的动态直接观察的证实。我们还建立了体外eNOS基因导入培养的血管内皮细胞，但不能建立体内移植修复受损内皮细胞的实验模型。5.本研究结果将为ABO血型不合肾移植抗体介导的排斥反应和已故供者肾移植缺血再灌注损伤的临床肾保存和再生治疗提供实验依据。

1. From the clinicopathological observation of antibody-mediated rejection in living donor ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation, We had analyzed and evaluated the process of the microcirculation injury and microvascular endothelial cell damage.2. We had established the GFP(+) to (-) rat bone marrow transplantation model and evaluated the role of The bone marrow derived endothelial progenitor cells in the regeneration of the injured microvascular Cells in renal ischemia-reperfusion injury.3. We have revealed that the bone marrow derived endothelial cells have migrated and recovered the injured endothelial cells in renal ischemia-reperfusion injury.It was approved not only by static immunohinstopathological method but also by dynamic direct observation by real-time phase contrast microscopy.4. We have also established in vitro eNOS gene transfection into cultured vascular endothelial cells, However, transplantation and restoration of injured endothelial cells by eNOS transfectant endothelial cells in "in vivo" experimental model could not be established.5.These result will be useful for the clinical renal preservation and regeneration therapy for antibody-mediated rejection in ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation.