Studies of ultra structures in 3-D on developmental mechanisms of biological crystals.

细胞膜冷冻超微切片术直到研究期结束时才能获得用于本研究目的的适当仪器。因此，我们仅使用甲基丙烯酸甲酯的方法通过包埋标本来制备标本。正如去年（2005年）提交的研究摘要中所提到的，我们使用的甲基丙烯酸甲酯单体具有高度挥发性，因此我们对这种树脂的聚合进行了许多新的设计方法。首先，我们将甲基丙烯酸异丙酯放入一个小的带密封盖的样品瓶中。进一步采用两步包埋法，即先将一半单体在瓶中聚合，然后包埋试样，再将试样置于聚合树脂上，再倒入剩余单体进行充分聚合。根据这种包埋方法，我们可以使标本漂浮在较大的树脂块中。聚合后，瓶子被打破， ...更多信息 树脂块，然后将试样锯出以制成合适尺寸的试样块。由于试块太大，不利于我们做更长的工作来获得实验试块，并且总是要注意不要错过试件的方向。本年度，我们尝试了在成型过程中保持标本方向偏离侧面的方式进行成型。在研究期结束时，无法解决将超薄切片放置在载玻片上进行后续脱包埋处理的问题。我们计划用环氧骨水泥将切片放在载玻片上，用单体冲洗，以制作不含树脂的组织。但我们可以找到任何合适的方法，我们完全可以踩在最初计划的初步研究，因为我们需要太多的时间来丢失这些问题。然而，在这四年里，我们团队的每一位研究者都在积极地进行这项研究，同时我们每个人都取得了报告中所描述的良好成果。少

The cryo-ultramicrotomy for cell membrane experiences could not have appropriate instrumentation for the aim of this research until the end of research period. So we made preparation of specimen by embedding specimen using only metyl-metacrylate method.As mentioned on research abstract proposed last year (2005), the monomer of metyl-metacrylate we use has highly volatile so we made much newly devised methods for polymerization of this resin. At first, we made to polymerize metyl-metacrylate in the small specimen bottle with tight cap. It could do polymerization with minimum volatilization.We made further to work it by two step embedding method, that is, half part of monomer was polymerized in the bottle before embedding specimen, then the specimen was set on the polymerized resin and pours rest of monomer for thoroughly polymerization. According to this embedding method, we could have the specimen floating in the larger resin block. After polymerization, the bottle was broken to have t … More he resin block, and then the specimen was sawed out to make suitable size of specimen block. Because the block is too large, it is disadvantage we have to make longer work for getting the experimental block, and always to watch not missing the specimen direction. In this year, we tried to shape blocks with keeping the specimen direction from the lateral surfaces on the way to the final shape.We could not solve in the end of research period the problem of putting the ultra-thin sections on a slide glass for later treatment of de-embedding. We had the plan that sections put on the slide glass with the epoxy cement were rinsed by monomer to make tissue without resin. But we could find any appropriate method for it.Totally we could step on the primary research scheduled in the beginning, because we needed too much time for losing those problems. However, for those four years, each investigator of our team worked eagerly on this research, and at the same time each of us achieved good results personally as described on the report. Less