Conservation of natural diversity of legume nodulation symbiotic genes using crop wild relatives core collection

1.植物-根瘤菌遗传资源的保护考察了日本冲绳县石垣岛、东帝汶、老挝和巴布亚新几内亚等地的植物-根瘤菌遗传资源，收集并保存了新的植物-根瘤菌遗传资源。2.植物-根瘤菌遗传资源多样性分析与温带和泰国大豆慢生根瘤菌相比，泰国豌豆慢生根瘤菌的nod基因RFLP谱变异较大，并产生了不同的结瘤因子。在序列分析的基础上，将泰国豌豆根瘤菌分为日本慢生型根瘤菌和日本石垣岛自然生境中采集的根瘤菌16S rRNA基因序列。发现了根瘤菌、慢生根瘤菌和中华根瘤菌。从试验田种植的四季豆和木豆(Cajanus Cajan)中分离到根瘤菌，并进行了…分析更多的西拉特罗(Macroptilum AttPurpureum)逃逸种群。从试验田及其自然栖息地的豌豆、木豆、木豆和野生豌豆中分离到慢生型根瘤菌。从蚕豆野生种群中分离到中华根瘤菌。3.寄主植物的多样性分析利用莲花NFR1和NFR5基因的序列信息，从小豆(Vigna Angularis)中分离到结瘤因子受体(NFR)基因。分离得到的Vigna NFR1样基因和Vigna NFR5样基因与莲花中相应基因的氨基酸序列同源性分别为63%和71%，并保持了LysM、跨膜和蛋白激酶的相同结构。基于azuki NFR-like基因的序列信息，已经从其他豌豆属植物中分离到了同源基因。对不同种的Vigna进行序列比较，发现其LysM结构域的氨基酸序列存在差异，表明在Vigna属内NFR类基因存在功能分化。较少

1.Conservation of plant-rhizobia genetic resourcesIshigaki island, Okinawa prefecture, Japan, East Timor, Laos and Papua New Guinea have been explored and new plant-rhizobia genetic resources have been collected and are conserved.2.Diversity analysis of rhizobiaDiversity of rhizobia strains from Thai Vigna host plants were analyzed. Thai Vigna Bradyrhizobium rhizobia were revealed to have much higher variation in RFLP profiles of Nod gene and produced diverse Nod factors compared with temperate and Thai soybean Bradyrhizobium. Based on sequence analysis, Thai Vigna rhizobia strains were classified into two groups corresponding to Bradyrhizobium japonicum and B.elkanii.16S rRNA gene sequencing of rhizobia strains collected from experimental field and natural habitat on Ishigaki island, Japan. revealed Rhizobium, Bradyrhizobium and Sinorhizobium rhizobia strains. Rhizobium strains were isolated from cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) grown in experimental fields an … More d an escaped population of siratro (Macroptilium atropurpureum). Bradyrhizobium strains were isolated from cowpea, pigeon pea, siratro and wild Vigna species in experimental fields and their natural habitat. Sinorhizobium strains were isolated from wild populations of Vigna marina. This is the first record of Sinorhizobium strains isolated in Japan.3.Diversity analysis of host plantsPutative Nod factor, receptor (NFR) genes have been isolated from azuki bean (Vigna angularis) using the sequence information of NFR1 and NFR5 genes of Lotus japonicus. Isolated Vigna NFR1-like and Vigna NFR5-like genes showed 63% and 71% amino acid sequence homology to counter part genes in Lotus and conserved the same structure of LysM, trans membrane and protein kinase. Based on the sequence information of azuki NFR-like genes, homologous genes have been isolated from other Vigna species. Comparison of the sequence of various Vigna species revealed amino acid sequence variation in LysM domain indicating the functional differentiation of NFR-like genes within the genus Vigna. Less