Stimulation of germination and storage of seeds of orchids that are native to Japan

许多兰科植物在全球范围内受到开发和从自然栖息地过度采集的威胁。对大多数原产于温带和亚热带地区的陆生兰花来说，种子离体人工繁殖是困难的。本研究揭示了对日本温带和亚热带地区原产兰花种子萌发的刺激作用。提出了这些兰花种子建立幼苗的可复制方案。对这些种的种子贮藏条件也进行了调查，估计它们是顽固的。为了这些种子的长期保存，建立了冷冻保存方法。

Many Orchidaceous species are threatened globally by development and over-collection from their natural habitats for horticultural purposes. Artificial propagation from seeds in vitro is difficult in most terrestrial orchids native to temperate and sub tropical regions. In the present study, stimulatory effects on the germination of seeds of orchids native to temperate and subtropical regions of Japan were revealed. Reproducible protocols for the seedling establishment from seeds of those orchids were proposed. The storage conditions for seed in those species were also investigated and they were estimated as recalcitrant. For the long-term storage of those seeds, cyropreservation methods were established.