Gene therapy for severe bacterial pneumonia and sepsis.

根据最近对急性肺损伤的研究，角质细胞生长因子（KGF）已被证明对各种有害刺激具有一定的保护作用，并促进上皮修复。因此，在这项研究中，我们研究了是否瞬时过表达KGF的腺病毒载体在肺内给药的KGF在小鼠高氧诱导的急性肺损伤减弱。目前的数据表明，腺病毒介导的KGF cDNA转移成功地在气道上皮细胞中表达高水平的KGF，并因此显著提高肺上皮细胞增殖，改善氧合，并降低死亡率。此外，存在最小的载体相关炎症。我们认为，KGF基因转导入肺是一个有前途的潜在治疗急性肺损伤，并将是一个有用的工具，再生研究。

On the basis of recent studies dealing with acute lung injury, keratinocyte growth factor (KGF) has been shown to have certain protective effects against various injurious stimuli and to promote epithelial repair. Thus, in this study, we examined whether transient overexpression of KGF in the lung by the intratracheal administration of KGF-expressing adenovirus vector attenuates hyperoxia-induced acute lung injury in mice. The present data show that the adenovirus-mediated transfer of KGF cDNA successfully expressed high level of KGF in airway epithelial cells, and consequently raised significant lung epithelial cell proliferation, improved oxygenation, and decreased mortality. Furthermore, there was minimal vector-associated inflammation. We suggest that KGF gene transduction into lungs is a promising potential treatment for acute lung injury and would be a useful tool for regeneration research.