Research of Development of Restorative Material Using Enamel regenerated and CAD/CAM

在牙科医学领域，由于牙周病和随年龄增长而导致的龋齿继续影响着大多数老年人的生活质量，因此需要提高生活质量。因此，追求牙科治疗的目标，对于最高水平的咬合维护、口腔卫生和美容修复是必不可少的。将组织工程的概念应用于传统牙科的牙齿再生是解决口腔修复中老年患者牙齿缺失问题的一种很有前途的生物学方法。在本研究中，我们研究了猪牙蕾来源的牙源性上皮细胞的培养以及通过层叠这些细胞层来复制釉质块。猪第三磨牙牙芽上皮细胞可传代2代并表达…将分离的牙源性上皮细胞接种于可生物降解的聚乙醇酸(PGA)纤维膜上，在含10%胎牛血清的D-MEM中培养一周。培养结束后，用扫描电子显微镜(SEM)和荧光显微镜(EM)对细胞膜进行观察。结果表明，上皮细胞在PGA膜上均匀分布和定植。然后，将10个上皮细胞定植的膜堆积在无菌大鼠的大网膜内。植入后4周取材，制作组织切片，苏木精-伊红(H-E)染色。光镜下的组织切片观察证实了膜间再生组织的存在。综上所述，我们的研究结果表明牙源性上皮细胞是通过使用可生物降解的PGA膜进行层积来实现组织再生的。进一步的全面研究将有必要阐明分化事件的顺序和釉质组织再生的细胞谱系，以便临床应用。较少

In the field of dental medicine, improvement of Quality of Life (QOL) is required because tooth loss due to periodontal disease and dental caries with progression of aging continues to affect most elderly at some time in their lives. Therefore, it is essential for occlusal maintenance, oral hygiene and esthetic restorations at the highest levels to pursue the goal of treatment at dentistry. Application of tooth regeneration using tissue engineering concepts for convemtional dentistry is a promising biological approach to solving problems of tooth loss in elderly patients in prosthodontics.We aimed at dental enamel regeneration using tissue engineering capable of clinical application. In the present study, we investigated the cultivation of odontogenic epithelial cells derived from porcine tooth buds and the reproduction of enamel bulk by stratification of these cell layers. Epithelial cells isolated from porcine tooth buds of third molar can be subcultured by 2 passages and expressed a … More melogenin which is specific for dental enamel, indicating that these cells have the characters of ameloblast progenitor.Moreover the isolated odontogenic epithelial cells were seeded onto biodegradable polyglycolic acid (PGA) fiber membranes and cultured in D-MEM containing 10% fetal bovine serum for a week. After culturing, membranes were observed by the scanning electron microscopy (SEM) and cell nuclear staining using fluorescent microscopy (EM). The result showed that epithelial cells were spread uniformly and colonilized on PGA membrane. Following that, ten membranes where epithelial cells colonilized were piled up and implanted into the omenta of the athymic rats. At 4 weeks after implantation, samples developed were dissected, prepared tissue sections and stained with hematoxylin and eosin (H-E). Regenerated tissues at inter-membrane were confirmed by observation of tissue sections by light microscopy.In conclusion, our findings imply that tissue regeneration was achieved by the stratification of cultured odontogenic epithelial cells using biodegradable PGA membranes. Further comprehensive studies will be necessary to clarify the sequence of differentiation events and the cell lineage of enamel tissue regeneration for clinical application. Less