The role of motile activity of mesenchymao stem cells in regeneration of musculoskeletal organs

我们在骨盆手术患者的骨髓中建立了10个间充质干细胞培养细胞系，发现它们分化为骨和软骨。自体血清在细胞增殖和移动能力方面优于胎牛血清。同样在动物模型中，一种特定的透明质酸作为干细胞的支架，对软骨再生有很好的作用，但这种作用不依赖于CD44。此外，自分泌运动因子（AMF）在周围滑膜干细胞诱导的半月板再生中发挥作用。另一方面，在骨和软组织肿瘤中，表达RNA信息而非蛋白与恶性相关，且蛋白受细胞内降解调控。这在肺癌中也有发现。发现AMF分子与聚adp核糖聚合酶家族14结合。这些结果从临床和基础的角度对阐明和促进间充质干细胞细胞运动的分子生物学具有重要意义。

We have established 10 cultured cell lines of mesenchymal stem cells from bone marrow in patients receiving a surgical treatment at pelvis, and found that they differentiate into bone and cartilage. For the culture autologous serum was superior to fetal bovine serum in the cell ability to proliferate and move. Also in an animal model, a specific hyaluronic acid was found to have excellent effect on cartilage regeneration as a scaffold for stem cells, but the effect being independent of CD44. Furthermore, Autocrine motility factor (AMF) play a role in meniscus regeneration induced by surrounding synovial stem cells. On the other hand, expression of RNA message rather than protein was related to malignancy in bone and soft tissue tumors, and the protein was regulated by intracellular degradation. This was also found in lung cancers. The AMF molecule was found to bind to poly(ADP-ribose) polymerase family 14. These results play an important role in the elucidation and promotion of molecular biology in cell motility of mesenchymal stem cell, from clinical and basic points of view.