Molecular dissection of regulatory mechanism that controls cell-type specific cell wall construction using XTH family of genes as a model system

木葡聚糖酶/水解酶(XTHs)是一类能够催化木葡聚糖分子间的接枝和/或木聚糖分子的内型水解酶。它们由拟南芥中的33个基因编码。虽然最近的研究已经揭示了植物中该家族单个成员在时间和空间上的特定表达谱，但它们的生物学作用仍有待阐明。为了确定这个基因家族中每个成员的作用，我们检查了每个拟南芥Xth基因被打乱的突变体的表型。AtXTH27基因的两个功能缺失突变体xth27-1和xth27-2在第三脉中显示出短形的管状分子，并减少了第一叶中的第三脉的数量。在突变体的成熟莲座叶中，也观察到黄色的类似病斑的斑点。通过将野生型XTH27基因导入xth27-1突变植株进行遗传互补，恢复了三级脉的数量，…病变完全消失。PXTH27：：GUS融合基因在丛生叶未成熟的管胞中广泛表达。在莲座状叶片中，AtXTH27基因的表达水平在叶片展开期间达到最高水平，此时导管分子正在伸长。这些结果表明，AtXTH27在拟南芥丛生叶中管状分子的产生过程中起着重要的作用。拟南芥Xth基因AtXTH17、18、19和20在系统发育上彼此关系密切，并且优先在根中表达。然而，它们在根中表现出不同的表达谱，对激素信号的反应也不同。为了研究它们在根生长中的功能，我们使用T-DNA插入系和RNAi植株检测了这些基因功能缺失突变体的表型。这些功能分析揭示了AtXTH18基因在初级根伸长中的主要作用。在四个Xth基因中，AtXTH18的mRNA表达水平最高。我们还利用AXR2/IAA7基因缺陷的突变体确定了这些基因的生长素信号通路，发现AtXTH19在根的伸长/成熟区的表达受AXR2/IAA7信号通路的控制。较少

Xyloglucan endotransglucosylases/hydrolases (XTHs) are a class of enzymes capable of catalyzing the molecular grafting between xyloglucans and/or the endotype hydrolysis of a xyloglucan molecule. They are encoded by 33 genes in Arabidopsis. Whereas recent studies have revealed temporally and spatially specific expression profiles for individual members of this family in plants, their biological roles are still to be clarified. To identify the role of each member of this gene family, we examined phenotypes of mutants in which each of the Arabidopsis XTH genes was disrupted.Two loss-of-function mutants for the AtXTH27 gene, xth27-1 and xth27-2 exhibited short-shaped tracheary elements in tertiary veins, and reduced the number of tertiary veins in the first leaf. In mature rosette leaves of the mutant, yellow lesion-mimic spots were also observed. Upon genetic complementation by introducing the wild-type XTH27 gene into xth27-1 mutant plants, the number of tertiary veins was restored, and … More the lesions disappeared completely. Extensive expression of the pXTH27::GUS fusion gene was observed in immature tracheary elements in the rosette leaves. The highest level of AtXTH27 mRNA expression in the rosette leaves was observed during leaf expansion, when the tracheary elements were elongating. These findings indicate that AtXTH27 plays an essential role during the generation of tracheary elements in the rosette leaves of Arabidopsis.The Arabidopsis thaliana XTH genes AtXTH17, 18, 19, and 20 are phylogenetically closely related to one another and are preferentially expressed in the roots. However, they exhibit different expression profiles within the root and respond to hormonal signals differently. To investigate their functions in root growth, we examined phenotypes of loss-of-function mutants for these genes using T-DNA insertion lines and RNAi plants. These functional analyses disclosed a principal role for the AtXTH18 gene in primary root elongation. Of the four XTH genes, AtXTH18 exhibits the highest level of mRNA expression. We also determined auxin-signaling pathways for these genes using a mutant with a defect in the AXR2/IAA7 gene and found that the expression of AtXTH19 in the elongation/maturation region of the root is under the control of the AXR2/IAA7 signaling pathway. Less