Reasonable recellularization of the decellularized pulmonary valve in vivo

背景和目标：尽管已经开发了各种技术来再细胞化同种异体/异种脱细胞心脏瓣膜和组织工程可生物降解支架，但是用于受体细胞再细胞化的脱细胞基质支架的适当条件和这些移植物的再生机制仍有待阐明。本研究的目的是从基质结构的角度寻找最佳的心脏瓣膜脱细胞技术，并在体内使用小猪模型评价受体细胞的再细胞化。适当的脱细胞技术=新开发的氢氧化铵-Triton法，即使是0.1%浓度的氢氧化铵，也可以完全脱细胞。氢氧化铵-曲拉通法虽然能较好地保持主要基质结构的弹性蛋白和胶原纤维，但大部分糖胺聚糖纤维几乎消失。关于迪 ...更多信息 脱细胞肺动脉瓣的体内再细胞化：将猪脱细胞单尖瓣移植物植入同种异体仔猪模型的颈内静脉，植入4周后收获。免疫组化结果显示，移植组织表面及内层可见α-SMA阳性细胞迁移，vWF阳性细胞覆盖移植组织表面，移植组织的基质结构似乎得以保留，但大量炎性细胞浸润。讨论：从保存基质组织的角度出发，我们开发了0.1%氢氧化铵-Triton法作为一种合适的脱细胞技术。这些关于体内植入的脱细胞肺动脉瓣的再细胞化的数据暗示受体细胞迁移到具有脱细胞基质支架的移植物中。对于移植物的合理再细胞化，需要进一步检查以排除炎性细胞浸润，并具有良好的血流动力学和耐久性，以便在未来的临床应用中更好地使用去细胞技术。少

Background and Objectives : Although various technologies to recellularize both allogenic/xenogenic decellularrized heart valves and tissue-engineered biodegradable scaffolds have been developed, adequate condition of the decellularized matrix scaffold for recellularization by recipient cells and mechanism of regeneration of these grafts remain to be elucidated. The purpose of this study was to find optimal decellularizing techniques for the heart valve from the point of the matrix structure and to evaluate the recellularization by recipient cells using piglet model in vivo.Adequate decellularization techniques= Newly developed ammonium hydroxide-triton method, even the 0.1% concentration of ammonium hydroxide, decellularized the heart valve tissue completely. Although the elastin and collagen fiber, which is the main matrix structure, was maintained with good condition by the ammonium hydroxide-triton method, most of glycosaminoglycan fiber has almost disappeared. Concerning on the di … More sappearance of glycosaminoglycan, further examination to preserve the glycosaminoglycan fiber will be required for better decellularization.Recellularization of the decellularized pulmonary valve in vivo : Porcine decellularized monocusp valve graft was implanted to the internal jugular vein of allogenic piglet model, and was harvested four weeks after implantation. Immunohistological examination showed that α-SMA positive cells migrated into the surface and inner layer of the graft tissue, and vWF positive cells covered the surface of it. Matrix structure of the graft seemed to be preserved, however, massive inflammatory cells infiltrated into the graft tissue.Discussion : We developed 0.1% ammonium hydroxide-triton method as an adequate decellularization technique from the point of matrix tissue preservation. These data on recellularization of the decellularized pulmonary valve implanted in vivo imply that recipient cells migrate into the graft with decellularized matrix scaffold. Concerning on reasonable recellularization of the graft, further examination to exclude the inflammatory cells infiltration and to function with good hemodynamics and durability will be required for better decellularization technology in future clinical use. Less