Analysis of new transposable elements involved in mutations of flower color

从龙胆属植物中分离并分析了新的转座因子。（1）在一株粉红色龙胆中发现一个插入在类黄酮3，5 '-羟化酶（F3' 5 'H）基因上的转座因子（dTgt 1）。该转座因子具有hAT型转座因子的特征，不编码任何蛋白质，因此被认为是一个非自主转座因子。表达分析表明，F3 '5'H转录本的积累减少，表明GshAT I的插入导致了蓝花龙胆的粉红色。2）通过简并PCR分离了龙胆花瓣中调控类黄酮生物合成的几个转录因子。其中，R2 R3 MYB基因，命名为GtMYB 3，被认为是参与调控后期花青素生物合成基因。序列分析表明，在白花龙胆品种的GtMYB 3基因组序列中插入。该插入片段命名为dTgs 2，长度为271 bp， ...更多信息 具有hA T型转座因子的特征。北方印迹分析表明，白花品种中GtMYB 3的信号比蓝花品种长。在其他白花龙胆品种中也发现了GtMYB 3基因中插入的其他转座因子，详细分析正在进行中。3）从龙胆悬浮培养细胞中分离到几个新的转座因子，并对其进行了转座分析。分离出hAT和En/Spm转座因子及LTR-型转座因子。使用悬浮培养细胞进行的水杨酸、茉莉酸和5-azadC诱导分析表明，这些处理可以激活其中一些转座因子。Southern杂交分析表明，这些转座因子属于龙胆属植物基因组中一个拷贝数较高的家族，这些转座因子的分离对龙胆属植物的基础和应用研究具有重要意义，我们将继续对龙胆属植物转座因子的研究。少

We isolated and analyzed new transposable elements from gentian plants (Gentiana triflora, G. scabra and their hybrids).1) A transposable element (dTgt1) inserted in flavonoid 3, 5'-hydroxylase (F3'5'H) gene was found in a pink-flowered gentian. This transposable element had a feature of hAT-type transposable element and did not encode any protein and thus considered as an nonautonomous one. Expression analysis showed that accumulation of F3'5'H transcripts decreased, suggesting that the insertion of GshAT I caused pink coloration from blue-flowered gentian.2) Several transcriptional factors that regulate flavonoid biosynthesis in gentian petals were isolated by degenerated PCR. Among them, an R2R3 MYB gene, designated as GtMYB3, was considered to be involved in the regulation of late anthocyanin biosynthetic genes. Sequence analysis showed an insertion in GtMYB3 genomic sequence of a white-flowered gentian cultivar. This insertion fragment designated as dTgs2 was 271 by in length and … More had a feature of hA T-type transposable element. Northern blot analysis showed that signal of GtMYB3 was longer in the white-flowered cultivars than blue-flowered cultivar. Other transposable elements inserted in GtMYB3 gene were also found in the another white-flowered gentian cultivars and the detailed analyses are in progress.3) Several new transposable elements were isolated from suspension-cultured cells of gentian and analyzed for their transposition. hAT and En/Spm transposable elements and LTR-type retorotransposable elements were isolated. Induction analysis by salicylic acid, jasmonic acid and 5-azadC using suspension-cultured cells showed that some of these transposable elements could be activated by the treatments. Southern blot analyses indicated that these transposable elements belong to members of a relatively high copy number family in the gentian genome.These isolated transposable elements in this study are useful for basic and applied studies and we are continuously studying the elucidation of the transposable elements in gentian plants. Less