Development of a in vivo screening method for evaluation prostate carcinogenic potential in chemicals

我们获得了三种致癌物-氨基- 1 -甲基-6-苯基咪唑[4,5-b]吡啶（PhIP）、3,2 '-二甲基-4-氨基联苯（DMAB）和镉处理的大鼠前列腺腹侧组织的基因表达谱。只有三个基因在这三种化学物质中普遍上调。在这些基因中，我们重点研究了肿瘤调节素，并对经化学物质处理的腹侧前列腺中肿瘤调节素的表达进行了免疫组化分析。癌调节素在前列腺细胞质中呈散发阳性。而除DMAB外，肿瘤调节素在未处理对照组和3种致癌化学物质组的表达均无差异。这些结果表明，oncompdulin表达分析可能不适合用于前列腺癌物的体内筛选方法。

We obtained gene expressions profiles of rat ventral prostate tissue treated with three kinds of carcinogens, -amino-l-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), 3, 2'-dimethyl-4-aminobiphenyl (DMAB)and cadmium. There were only three genes that was commonly up regulated in the 3 chemicals. Among the genes, we focused on oncomodulin and performed immunhistochemicl analysis for expression of oncomodulin in ventral prostates treated with the chemicals. Oncomodulin demonstrated sporadic positivity in cytoplasms of the prostate glands. However, there were no differences for expressions of oncomodulin between non-treated control group tissues and the 3 carcinogenic chemical groups, except for DMAB. There results indicated that the oncompdulin expression analysis may not be suitable for a in vivo screening method for prostate carcinogens.