Study on the mode of human parvovirus B19 infection

人类细小病毒B19(Human ParvoVirus B19，B19)是引起儿童感染性红斑和先天性慢性溶血性贫血患者再生障碍性疾病的病原体。B19被认为只有一个免疫血清型的人，他们患有由B19终身免疫引起的疾病，通常不会再次感染。作为一种罕见的情况，我们遇到了一位初次感染3年后似乎再次感染的患者，这是一个罕见的病例。对初次感染和继发感染患者的血清样本进行分析比较，发现初次感染和继发感染的基因组类型没有差异。在初次感染后，潜伏的B19感染可能会持续。潜伏性B19感染的病理机制将被研究，在这种情况下，可能有一种特殊的菌株，易于潜伏性感染。从1995年到2005年，我检测了9000份血清样本。30份标本B19DNA呈阳性。目前正在对核苷酸序列进行分析。我们将研究核苷酸和症状之间的联系。分析后，将对含有B19序列的胞浆进行人工突变，并通过研究B19各自的mRNA转录本来研究感染模式。现在我在上面所示的潜伏感染病例中发现了胸腺嘧啶核苷到腺嘌呤的特殊突变。另一项分析目前正在进行中。

Human parvovirus B19 (B19) is known as causative agents of erythema infectiosum in children and aplastic crisis in patients with congenital chronic hemolytic anemia. B19 is believed to have a single immunoserotype individuals who have suffered a disease induced by B19 aquire lifelong immunity and won't usually be reinfected. As a rare occurrence, several patients with repeated B19 infection have been reported We encountered a patient who appeared to have reinfection 3 years after the primary infection. There was no difference in genome type between the primary and the secondary infection when serum samples from our patient who seemed to have a reinfection were analyzed and compared. Latent B19 infection may persist after a primary infection. The pathological mechanism of a latent B19 infection involved in such cases will be studied There may be a special strain which predisposes to latent infections. I have examined 9,000 serum samples from 1995 to 2005. Thirty samples were positive for B19 DNA. The nudeotides sequences are now being analyzed. The association between the nucleotides and symtoms are going to be examined. After this analysis, the artificial mutation will be made in the plasmide with B19 sequences and the mode of infection will be studied by investigating for respective mRNA transcripts of B19. Now I have found the special mutation of Thymidine to Adenine at 1969nt in the case of latent infection shown above. The other analysis is now being performed.