Diagnostic demonstration of telomere measurement anaplastic cancer arose from differentiated cancer in the thyroid by tissue quantitative fluorescence in situ hybridization

我们开发了一种利用组织定量荧光原位杂交(Q-FISH)来评估端粒长度的新方法。我们的方法可以用来区分食道病变的良恶性组织(肿瘤学，2006)。我们用Q-FISH技术检测乳头状癌和正常甲状腺组织的端粒长度。甲状腺乳头状癌细胞的平均TCR明显低于滤泡上皮细胞和成纤维细胞(P&lt；0.05)。晚期乳头状癌T2(>2 cm)和NLb(淋巴结转移侧方面积)以上的端粒长度明显低于T2和NLb以下的早期乳头状癌。这些结果表明，我们的TCR方法可以用于区分晚期和早期甲状腺癌。另一方面，人类最具侵袭性的恶性肿瘤之一，间变性甲状腺癌(Atc)预后极差，可能是…。更多的是因为它的基因组不稳定。我们推测，在ATC中观察到的非常快速的细胞周转可能加速了与肿瘤细胞恶性相关的端粒缩短和染色体不稳定性。为了比较和测量间变性甲状腺癌细胞株OCUT-1的染色体畸变率和端粒缩短，我们应用了定量荧光原位杂交(Q-FISH)技术。OCUT-1细胞表现出几种染色体异常，但有一个接近正常的46，XX染色体组，这使得分析核型变得容易。核型为50，XX，+7，+11，der(11)t(3；11)(q23；q23)x2，del(12)(p11.2p12)，+20，+1mar。我们仔细分析了OCUT-1核型异常与每条染色体上的端粒缩短和亚端粒的表达有关。异常染色体der(11)t(3；11)(q23；q23)x2上的亚端粒在11p上也有信号丢失，但在细胞遗传学正常的7、20三体和异常的del(12)染色体上未见信号丢失(p11.2p12)。3q的亚端粒有8个信号，其中一对保留在3q上，另一对保留在异常的11p上。我们的发现表明，端粒缩短和亚端粒丢失与该未分化甲状腺癌细胞系的遗传不稳定性有关。较少

We have developed a novel method for evaluating telomere length using tissue quantitative fluorescence in situ hybridization (Q-FISH). Our method can be used to distinguish between benign and malignant tissue in esophageal lesions (Oncology, 2006). We demonstrated to evaluate telomere length in papillary carcinoma and normal thyroid tissue by Q-FISH. The average TCR in the papillary carcinoma cell was significantly lower than that in the follicular epithelial cell and fibroblast cells in the thyroid (P<0.05). The advanced papillary carcinoma of more than T2 (more than 2 cm) and Nlb (lateral area of metastasis of lymph-node) was significantly lower telomere length than the early papillary carcinoma of less than T2 and Nlb. These results suggested our TCR method can be used to distinguish between advanced and early carcinoma of the thyroid. On the other hand, one of the most aggressive human malignancies, anaplastic thyroid carcinoma (ATC), has an extremely poor prognosis that may be exp … More lained by its genomic instability. We hypothesized that the very rapid cell turnover observed in ATC might accelerate telomere shortening and chromosomal instability associated with tumor cell malignancy. To compare and measure chromosomal aberrations and telomere shortening in an anaplastic thyroid cancer cell line, OCUT-1, we applied quantitative fluorescence in situ hybridization (Q-FISH) techniques. OCUT-1 cells display several chromosomal abnormalities, but have a near-normal chromosome complement of 46, XX, making it easy to analyze the karyotype. The karyotype showed 50, XX, +7, +11, der(11)t(3 ; 11)(q23 ; q23)x2, del(12)(p11.2p12), +20, +1mar. We analyzed carefully the abnormalities in karyotype of OCUT-1 associated with telomere shortening on each chromosomes and expression of subtelomeres. Subtelomeres on the abnormal chromosome der(11)t(3 ; 11)(q23 ; q23)x2 also showed loss of signals on 11p, but there was no loss of signals in the cytogenetically normal trisomies 7 and 20 or the abnormal chromosome del(12)(p11.2p12). Subtelomeres of 3q had eight signals, one pair remaining in place on 3q and another pair on the abnormal 11p. Our findings suggest that telomere shortening and subtelomere loss are correlated with genetic instability in this anaplastic thyroid carcinoma cell line. Less