DELOPMENT OF THREE-DIMENSIONAL ELECTROPHORETIC MICROSC OPE AND ITS APPLICATION TO SOFT MATERIALS

在本研究中，我们测量了分布在具有复杂内部结构的软质材料中的亚微米大小的胶体颗粒在外力作用下的波动和位移。从这些响应中，我们可以得到这些复杂系统的局部动力学。为此，我们新引入红外激光作为陷光，并将其分成两束，研制了双光束激光镊子。在这种情况下，利用两轴声光学衍射系统控制陷阱光的聚焦位置，实现了样品侧在10微米尺度范围内的二维高速运算。我们用荧光显微镜组装了这种光学镊子。此外，不仅实现了二维方向，而且还实现了激光的聚焦位置，在使用压电级的情况下，可以在三维方向上控制激光的位置，并使用通常的手动级作为显微镜的级，实现了系统在100nm深度方向上的扫描精度。我们研究了各种软质材料的局部力学物理性质，包括粘弹性测量和相互作用的直接测量。(1)成功地直接测量了向列液晶中分布的胶体颗粒间的长程和各向异性作用力。(2)我们成功地直接测量了作为活细胞模型系统的脂质体的表面张力和弯曲刚度。

In this research, we have measured the fluctuation and the displacement induced by an external force of the submicron-sized colloidal particles distributed in a soft material with a complex internal structure. From these responses, we can obtain the local dynamics of these complex systems.For this, we developed two beam laser tweezers by newly introducing IR laser as trap light, and dividing it into two. In this case, the focus position of the trap light was controlled using two axis sound optics diffraction system and a high-speed operation was achieved in two dimensions within the range of ten-micron scale in the sample side. We built such optical tweezers was assembled by using the fluorescence microscope. In addition, not only the direction of two dimensions but also the focus position of the laser light was achieved in using the piezo stage where the position was able to control in three dimensions together with a usual manual stage as a stage for the microscope and the system that was able to scan in accuracy in direction 100nm of depth was achieved.We have studied the local mechanics physical properties including the viscoelasticity measurement and the direct measurement of the interaction in a variety of soft materials. (1) We succeeded in the direct measurement of the long-ranged and anisotropic interparticle force between colloidal particles distributed in the nematic liquid crystal. (2) We have succeeded in the direct measurement of the surface tension and the bending rigidity of a liposome as a model system of a living cell.