Development of siRNA Gene Therapy in Animal Models of Venous Thromboembolism

骨科手术，特别是全膝关节和全髋关节置换术，被认为是围手术期静脉血栓栓塞症的危险因素。我们的研究目的是评估加速的致炎细胞相互作用以及随后产生的组织因子和CD40配体在静脉血栓栓塞症发病机制中的重要作用。研究表明，在全膝关节置换术围手术期，血小板、白细胞和内皮细胞的活性及其相互作用增强，特别是患者下半身的静脉血，从而增强了血液的凝固性。在下一个项目中，我们证明了在全膝关节置换术围手术期持续输注米力农有可能通过抑制血小板内p38MAPK、1VIEK/ERK和PI3K/AKT信号通路来防止加速的血细胞相互作用和高凝状态。这些内容将提交给《科学》杂志。

Orthopedic surgery, especially total knee and total hip arthroplasty, is considered a risk factor for perioperative venous thromboembolism. Our aim of the study was to evaluate how accelerated inflammatogenic cellular interactions and the subsequent production of tissue factor and CD40 ligand play an important role in the pathogenesis of venous thromboembolism. We have demonstrated that platelet, leukocyte and endothelium activities as well as their interactions are enhanced during the perioperative period of total knee arthroplasty, particularly in venous blood from the lower half of the bodies of patients, which consequently augments blood coagulability in the research paper. (J Thromb Haemost.5, 738-45.2007)In the next project, we have demonstrated continuous perioperative milrinone infusion has the potential to prevent accelerated blood cell interactions and hypercoagulablility during the perioperative period of total knee arthroplasty via inhibition of intraplatelet p38 MAPK, 1VIEK/ERK, and PI3K/Akt signaling pathway. These contents will be submitted to the science journal.